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Literature summary for 1.5.1.3 extracted from
- Characterization and amino acid sequence of Neisseria gonorrhoeae dihydrofolate reductase (1984), J. Biol. Chem., 259, 12291-12298.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer | Neisseria gonorrhoeae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Neisseria gonorrhoeae |  |
| pyrimethamine | - |
Neisseria gonorrhoeae | |
| trimethoprim | - |
Neisseria gonorrhoeae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
7,8-dihydrofolate | - |
Neisseria gonorrhoeae | |
| 0.01 | - |
NADPH | - |
Neisseria gonorrhoeae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17300 | 18300 | both forms, nondenaturing PAGE | Neisseria gonorrhoeae |
| 18000 | - |
SDS-PAGE | Neisseria gonorrhoeae |
| 173000 | - |
1 * 173000, both forms, SDS-PAGE | Neisseria gonorrhoeae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neisseria gonorrhoeae | - |
2 forms, not genetically determined isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neisseria gonorrhoeae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH + H+ | - |
Neisseria gonorrhoeae | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 173000, both forms, SDS-PAGE | Neisseria gonorrhoeae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Neisseria gonorrhoeae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Neisseria gonorrhoeae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00002 | - |
trimethoprim | - |
Neisseria gonorrhoeae | |
| 0.0003 | - |
pyrimethamine | - |
Neisseria gonorrhoeae | |
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Release 2023.1 (January 2023)
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