Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM | Enterococcus faecium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cs+ | activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM | Enterococcus faecium | |
K+ | activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM | Enterococcus faecium | |
Li+ | activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM | Enterococcus faecium | |
methopterin | - |
Enterococcus faecium | |
Na+ | activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM | Enterococcus faecium | |
NH4+ | activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM | Enterococcus faecium | |
p-mercuribenzoate | mutant enzyme: no effect, wild type: inhibition | Enterococcus faecium | |
phenylmercuric acetate | mutant enzyme: no effect, wild type enzyme: inhibition | Enterococcus faecium | |
Rb+ | activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM | Enterococcus faecium | |
Urea | inactivation | Enterococcus faecium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM | Enterococcus faecium | |
K+ | activation of mutant type enzyme activity at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM | Enterococcus faecium | |
Li+ | activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM | Enterococcus faecium | |
Na+ | activation of mutant type enzyme 0.2 mM, decrease of wild type enzyme activity above 0.1 mM | Enterococcus faecium | |
Rb+ | activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM | Enterococcus faecium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
velocity sedimentation, mutant type | Enterococcus faecium |
28000 | - |
wild type, gel filtration and velocity sedimentation | Enterococcus faecium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | - |
2 forms termed mutant type and wild-type enzyme in a single organism | - |
Enterococcus faecium | - |
var. durans | - |
Enterococcus faecium | - |
amethopterin-resistant strain | - |
Enterococcus faecium A | - |
var. durans | - |
Purification (Comment) | Organism |
---|---|
2 forms: mutant-type and wild type enzyme in a single organism | Enterococcus faecium |
amethopterin-resistant strain | Enterococcus faecium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
285 | - |
- |
Enterococcus faecium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Enterococcus faecium | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Enterococcus faecium A | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | - |
Enterococcus faecium | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | - |
Enterococcus faecium A | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
folate | mutant type | Enterococcus faecium | |
15 | - |
7,8-dihydrofolate | mutant type | Enterococcus faecium | |
133 | - |
7,8-dihydrofolate | wild-type | Enterococcus faecium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
folate, mutant type enzyme | Enterococcus faecium |
5.8 | - |
wild type, mutant type | Enterococcus faecium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Enterococcus faecium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Enterococcus faecium |