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Literature summary for 1.5.1.3 extracted from
- Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei (1972), Biochemistry, 11, 1018-1023.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Urea | slight activation | Lacticaseibacillus casei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Lacticaseibacillus casei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| organic mercurials | no activation | Lacticaseibacillus casei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14900 | - |
gel filtration, ultracentrifugation, isoenzyme I | Lacticaseibacillus casei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
isozymes I and II | - |
| Lacticaseibacillus casei | - |
amethopterin-resistant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozymes I and II | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Lacticaseibacillus casei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 14900, isozyme I, SDS-PAGE | Lacticaseibacillus casei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
isozyme I | Lacticaseibacillus casei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
7,8-dihydrofolate | isozyme I | Lacticaseibacillus casei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
isozyme I | Lacticaseibacillus casei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | isozymes I and II | Lacticaseibacillus casei | |
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Release 2023.1 (January 2023)
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