Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Clostridium botulinum |
Protein Variants | Comment | Organism |
---|---|---|
D114A | severe reduction (higher than 95%) in enzymatic activity | Clostridium botulinum |
E13A | severe reduction (higher than 95%) in enzymatic activity | Clostridium botulinum |
H92L | severe reduction (higher than 95%) in enzymatic activity | Clostridium botulinum |
K71R | severe reduction (higher than 95%) in enzymatic activity | Clostridium botulinum |
R15K | severe reduction (higher than 95%) in enzymatic activity | Clostridium botulinum |
W90S | severe reduction (higher than 95%) in enzymatic activity | Clostridium botulinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
pyruvate | pH 7.5, temperature not specified in the publication, Vmax: 0.9 micromol/min/mg protein | Clostridium botulinum | |
2.95 | - |
NADPH | pH 7.5, temperature not specified in the publication | Clostridium botulinum | |
29.5 | - |
L-ornithine | pH 7.5, temperature not specified in the publication | Clostridium botulinum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35850 | - |
calculated from cDNA | Clostridium botulinum |
36000 | - |
SDS-PAGE | Clostridium botulinum |
125000 | - |
gel filtration | Clostridium botulinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | A5HZ59 | UniProt | - |
Purification (Comment) | Organism |
---|---|
using anion-exchange and phosphocellulose P-11 chromatography | Clostridium botulinum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.6 | - |
pH 7.5, temperature not specified in the publication | Clostridium botulinum |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, freezing and thawing results in loss of all enzymatic activity | Clostridium botulinum |
-20°C, 25% (NH4)2SO4, several months, no loss in activity | Clostridium botulinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-N-acetylornithine + pyruvate + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
D-lysine + pyruvate + NADPH + H+ | enzyme also reacts with D-isomer | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
D-ornithine + pyruvate + NADPH + H+ | enzyme also reacts with D-isomer | Clostridium botulinum | N5-(L-1-carboxyethyl)-D-ornithine + NADP+ + H2O | - |
? | |
L-lysine + pyruvate + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
L-ornithine + 2-oxo-butanoic acid + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
L-ornithine + 3-fluoropyruvic acid + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
L-ornithine + 3-mercaptopyruvic acid + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
L-ornithine + glyoxylic acid + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
L-ornithine + methylglyoxal + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
L-ornithine + oxaloacetic acid + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
L-ornithine + pyruvate + NADPH + H+ | highest reaction rate | Clostridium botulinum | N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O | - |
? | |
N2-methylornithine + pyruvate + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
N6-hydroxy-L-lysine + pyruvate + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
O-(2-aminoethyl)-L-serine + pyruvate + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? | |
S-(2-aminoethyl)-L- cysteine + pyruvate + NADPH + H+ | weak reaction rate | Clostridium botulinum | ? + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | in solution, gel filtration | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
bCEOS | - |
Clostridium botulinum |
CBO0515 | - |
Clostridium botulinum |
N5-(carboxyethyl) ornithine synthase | - |
Clostridium botulinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Clostridium botulinum |