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show all sequences of 1.5.1.24

N5-(L-1-carboxyethyl)-L-ornithine synthase: Physical and spectral characterization of teh enzyme and its unusual low pKa fluorescent tyrosine residues

Sackett, D.L.; Ruvinov, S.B.; Thompson, J.; Protein Sci. 8, 2121-2129 (1999)

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
industrially important organism used widely as a starter in the dairy industry
Lactococcus lactis
Cloned(Commentary)
Commentary
Organism
ceo gene encoding N5-(carboxyethyl)ornithine synthase isolated from transposon Tn5306 of Lactococcus lactis K1 cloned and sequenced, expression in Escherichia coli CEO571; gene ceo, encoding the tetrameric enzyme, cloned and sequenced, plasmid p493 containing ceo transformed into Escherichia coli TG1
Lactococcus lactis
Crystallization (Commentary)
Crystallization
Organism
-
Lactococcus lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35320
-
deduced from amino acid sequence
Lactococcus lactis
37000
-
4 * 37000, SDS-PAGE
Lactococcus lactis
137000
-
XLA
Lactococcus lactis
140000
-
native PAGE
Lactococcus lactis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme purified from the overproducing strain Escherichia coli CEO571
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + pyruvate + NADPH
-
392178
Lactococcus lactis
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
ir
Subunits
Subunits
Commentary
Organism
tetramer
4* 35355, MALDI-MS; 4 * 37000, SDS-PAGE
Lactococcus lactis
Application (protein specific)
Application
Commentary
Organism
nutrition
industrially important organism used widely as a starter in the dairy industry
Lactococcus lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
ceo gene encoding N5-(carboxyethyl)ornithine synthase isolated from transposon Tn5306 of Lactococcus lactis K1 cloned and sequenced, expression in Escherichia coli CEO571; gene ceo, encoding the tetrameric enzyme, cloned and sequenced, plasmid p493 containing ceo transformed into Escherichia coli TG1
Lactococcus lactis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Lactococcus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35320
-
deduced from amino acid sequence
Lactococcus lactis
37000
-
4 * 37000, SDS-PAGE
Lactococcus lactis
137000
-
XLA
Lactococcus lactis
140000
-
native PAGE
Lactococcus lactis
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme purified from the overproducing strain Escherichia coli CEO571
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + pyruvate + NADPH
-
392178
Lactococcus lactis
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4* 35355, MALDI-MS; 4 * 37000, SDS-PAGE
Lactococcus lactis
Other publictions for EC 1.5.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712294
Thompson
The gene CBO0515 from Clostrid ...
Clostridium botulinum
J. Bacteriol.
192
1151-1155
2010
-
-
1
-
6
-
-
3
-
-
3
-
-
2
-
-
1
-
-
-
1
2
15
1
-
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1
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1
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6
-
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3
-
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3
-
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-
1
-
-
1
2
15
1
-
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1
-
-
-
-
-
-
-
-
-
392177
Ruvinov
Tetrameric N5-(L-1-carboxyethy ...
Lactococcus lactis
Arch. Biochem. Biophys.
371
115-123
1999
-
1
-
-
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1
-
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-
-
13
-
-
1
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1
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-
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2
1
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1
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1
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1
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1
-
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1
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-
-
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13
-
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1
-
-
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
392178
Sackett
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
Protein Sci.
8
2121-2129
1999
-
1
1
1
-
-
-
-
-
-
4
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
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4
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392176
Donkersloot
Cloning, expression, sequence ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
270
12226-12234
1995
-
2
1
-
-
-
-
-
-
-
1
-
-
7
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
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-
-
-
-
-
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-
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1
-
-
-
-
1
-
-
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4
-
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-
-
-
-
-
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-
-
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-
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-
392175
Miller
Enzymatic synthesis and charac ...
Lactococcus lactis
Amino Acids
6
189-198
1994
-
1
1
-
-
-
2
1
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
1
1
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
392174
Thompson
Transposon-encoded sucrose met ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
266
14573-14579
1991
-
-
1
-
-
-
-
-
-
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7
-
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-
-
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2
-
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1
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2
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-
392173
Donkersloot
Simultaneous loss of N5-(carbo ...
Lactococcus lactis
J. Bacteriol.
172
4122-4126
1990
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392172
Thompson
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
J. Biol. Chem.
264
9592-9601
1989
-
-
-
-
-
1
1
4
-
-
5
-
-
1
-
-
1
-
-
-
1
1
3
2
-
1
2
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
-
-
5
-
-
-
-
1
-
-
1
1
3
2
-
1
2
-
1
1
-
-
-
-
-
-
-
-