BRENDA - Enzyme Database show
show all sequences of 1.5.1.24

Tetrameric N5-(L-1-carboxyethyl)-L-ornithine synthase: Guanidine-HCl-induced unfolding and a low temperature requirement for refolding

Ruvinov, S.B.; Thompson, J.; Sackett, D.L.; Ginsburg, A.; Arch. Biochem. Biophys. 371, 115-123 (1999)

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
industrially important organism used widely as a starter in the dairy industry
Lactococcus lactis
General Stability
General Stability
Organism
enzymatic activity of CEOS is lost at guanidine-HCl concentrations from 0.5-1.2 M
Lactococcus lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34200
-
4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl
Lactococcus lactis
34700
-
4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl
Lactococcus lactis
34900
-
4 * 34900, sedimentation equilibrium, 20°C
Lactococcus lactis
35300
-
4 * 35300
Lactococcus lactis
35320
-
deduced from amino acid sequence
Lactococcus lactis
38600
-
4 * 38600, sedimentation equilibrium, 20°C
Lactococcus lactis
127500
-
sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl
Lactococcus lactis
135900
-
sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl
Lactococcus lactis
136900
-
sedimentation equilibrium at 20°C
Lactococcus lactis
138900
-
sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl
Lactococcus lactis
141300
-
gel filtration, analytical ultracentrifugation, sedimentation equilibrium
Lactococcus lactis
145700
-
sedimentation equilibrium at 4°C
Lactococcus lactis
146700
-
sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl
Lactococcus lactis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme purified from the overproducing strain Escherichia coli CEO571
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + pyruvate + NADPH
pyruvic acid serves as an effective keto substrate for the condensation reaction
392177
Lactococcus lactis
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
ir
lysine + pyruvate + NADPH
-
392177
Lactococcus lactis
N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
-
-
ir
Subunits
Subunits
Commentary
Organism
tetramer
4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl; 4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl; 4 * 34900, sedimentation equilibrium, 20°C; 4 * 35300; 4 * 38600, sedimentation equilibrium, 20°C
Lactococcus lactis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
heating CEOS in absence of guanidine-HCl produces irreversible denaturation and loss in activity
Lactococcus lactis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Lactococcus lactis
Application (protein specific)
Application
Commentary
Organism
nutrition
industrially important organism used widely as a starter in the dairy industry
Lactococcus lactis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Lactococcus lactis
General Stability (protein specific)
General Stability
Organism
enzymatic activity of CEOS is lost at guanidine-HCl concentrations from 0.5-1.2 M
Lactococcus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34200
-
4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl
Lactococcus lactis
34700
-
4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl
Lactococcus lactis
34900
-
4 * 34900, sedimentation equilibrium, 20°C
Lactococcus lactis
35300
-
4 * 35300
Lactococcus lactis
35320
-
deduced from amino acid sequence
Lactococcus lactis
38600
-
4 * 38600, sedimentation equilibrium, 20°C
Lactococcus lactis
127500
-
sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl
Lactococcus lactis
135900
-
sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl
Lactococcus lactis
136900
-
sedimentation equilibrium at 20°C
Lactococcus lactis
138900
-
sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl
Lactococcus lactis
141300
-
gel filtration, analytical ultracentrifugation, sedimentation equilibrium
Lactococcus lactis
145700
-
sedimentation equilibrium at 4°C
Lactococcus lactis
146700
-
sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl
Lactococcus lactis
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme purified from the overproducing strain Escherichia coli CEO571
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + pyruvate + NADPH
pyruvic acid serves as an effective keto substrate for the condensation reaction
392177
Lactococcus lactis
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
ir
lysine + pyruvate + NADPH
-
392177
Lactococcus lactis
N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl; 4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl; 4 * 34900, sedimentation equilibrium, 20°C; 4 * 35300; 4 * 38600, sedimentation equilibrium, 20°C
Lactococcus lactis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
heating CEOS in absence of guanidine-HCl produces irreversible denaturation and loss in activity
Lactococcus lactis
Other publictions for EC 1.5.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712294
Thompson
The gene CBO0515 from Clostrid ...
Clostridium botulinum
J. Bacteriol.
192
1151-1155
2010
-
-
1
-
6
-
-
3
-
-
3
-
-
2
-
-
1
-
-
-
1
2
15
1
-
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1
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1
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6
-
-
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3
-
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3
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1
-
-
1
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392177
Ruvinov
Tetrameric N5-(L-1-carboxyethy ...
Lactococcus lactis
Arch. Biochem. Biophys.
371
115-123
1999
-
1
-
-
-
1
-
-
-
-
13
-
-
1
-
-
1
-
-
-
-
-
2
1
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1
-
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-
-
1
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1
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1
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1
-
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-
-
-
-
13
-
-
-
-
1
-
-
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
392178
Sackett
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
Protein Sci.
8
2121-2129
1999
-
1
1
1
-
-
-
-
-
-
4
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392176
Donkersloot
Cloning, expression, sequence ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
270
12226-12234
1995
-
2
1
-
-
-
-
-
-
-
1
-
-
7
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392175
Miller
Enzymatic synthesis and charac ...
Lactococcus lactis
Amino Acids
6
189-198
1994
-
1
1
-
-
-
2
1
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
1
1
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392174
Thompson
Transposon-encoded sucrose met ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
266
14573-14579
1991
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392173
Donkersloot
Simultaneous loss of N5-(carbo ...
Lactococcus lactis
J. Bacteriol.
172
4122-4126
1990
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392172
Thompson
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
J. Biol. Chem.
264
9592-9601
1989
-
-
-
-
-
1
1
4
-
-
5
-
-
1
-
-
1
-
-
-
1
1
3
2
-
1
2
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
-
-
5
-
-
-
-
1
-
-
1
1
3
2
-
1
2
-
1
1
-
-
-
-
-
-
-
-