BRENDA - Enzyme Database
show all sequences of 1.5.1.24

N5-(L-1-carboxyethyl)-L-ornithine:NADP+ oxidoreductase from Streptococcus lactis. Purification and partial characterization

Thompson, J.; J. Biol. Chem. 264, 9592-9601 (1989)

Data extracted from this reference:

General Stability
General Stability
Organism
addition of 20% glycerol stabilizes the enzyme
Lactococcus lactis
Inhibitors
Inhibitors
Commentary
Organism
Structure
N5-(L-1-carboxyethyl)-L-ornithine
-
Lactococcus lactis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0066
-
NADPH
-
Lactococcus lactis
0.15
-
pyruvate
-
Lactococcus lactis
3.3
-
ornithine
-
Lactococcus lactis
18.2
-
lysine
-
Lactococcus lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
38000
immunoblot analysis
Lactococcus lactis
38000
-
2 * 38000, SDS-PAGE; 4 * 38000, SDS-PAGE
Lactococcus lactis
39200
-
calculated from amino acid sequence
Lactococcus lactis
78000
-
gel filtration
Lactococcus lactis
150000
-
PAGE, hight pressure liquid chromatography, gel filtration
Lactococcus lactis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
-
strains S.lactis K1 and 133; subsp. lactis K1, formerly Streptococcus lactis K1
-
Purification (Commentary)
Commentary
Organism
-
Lactococcus lactis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
40.5
-
-
Lactococcus lactis
Storage Stability
Storage Stability
Organism
-20°C, no significant loss of activity detected after 2 months of storage
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + pyruvate + NADPH
pyruvic acid serves as an effective keto substrate for the condensation reaction
392172
Lactococcus lactis
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
ir
lysine + pyruvate + NADPH
-
392172
Lactococcus lactis
N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
-
-
ir
ornithine + 3-fluoropyruvate + NADPH
slight activity observed, about 15% of the rate found with pyruvate
392172
Lactococcus lactis
N6-(1-carboxy-2-fluoroethyl)ornithine + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Lactococcus lactis
tetramer
4 * 38000, SDS-PAGE
Lactococcus lactis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
50
-
Lactococcus lactis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable to heating for 10 min
Lactococcus lactis
60
-
rapidly inactivated by heating at
Lactococcus lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Lactococcus lactis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
-
Lactococcus lactis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
high affinity for NADPH, NADH cannot replace NADPH, but nicotinamide hypoxanthine dinucleotide phosphate, reduced form, is an effective substitute for the natural cofactor
Lactococcus lactis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
high affinity for NADPH, NADH cannot replace NADPH, but nicotinamide hypoxanthine dinucleotide phosphate, reduced form, is an effective substitute for the natural cofactor
Lactococcus lactis
General Stability (protein specific)
General Stability
Organism
addition of 20% glycerol stabilizes the enzyme
Lactococcus lactis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
N5-(L-1-carboxyethyl)-L-ornithine
-
Lactococcus lactis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0066
-
NADPH
-
Lactococcus lactis
0.15
-
pyruvate
-
Lactococcus lactis
3.3
-
ornithine
-
Lactococcus lactis
18.2
-
lysine
-
Lactococcus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
38000
immunoblot analysis
Lactococcus lactis
38000
-
2 * 38000, SDS-PAGE; 4 * 38000, SDS-PAGE
Lactococcus lactis
39200
-
calculated from amino acid sequence
Lactococcus lactis
78000
-
gel filtration
Lactococcus lactis
150000
-
PAGE, hight pressure liquid chromatography, gel filtration
Lactococcus lactis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lactococcus lactis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
40.5
-
-
Lactococcus lactis
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, no significant loss of activity detected after 2 months of storage
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + pyruvate + NADPH
pyruvic acid serves as an effective keto substrate for the condensation reaction
392172
Lactococcus lactis
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
ir
lysine + pyruvate + NADPH
-
392172
Lactococcus lactis
N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
-
-
ir
ornithine + 3-fluoropyruvate + NADPH
slight activity observed, about 15% of the rate found with pyruvate
392172
Lactococcus lactis
N6-(1-carboxy-2-fluoroethyl)ornithine + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Lactococcus lactis
tetramer
4 * 38000, SDS-PAGE
Lactococcus lactis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
50
-
Lactococcus lactis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable to heating for 10 min
Lactococcus lactis
60
-
rapidly inactivated by heating at
Lactococcus lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Lactococcus lactis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9
-
Lactococcus lactis
Other publictions for EC 1.5.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712294
Thompson
The gene CBO0515 from Clostrid ...
Clostridium botulinum
J. Bacteriol.
192
1151-1155
2010
-
-
1
-
6
-
-
3
-
-
3
-
-
2
-
-
1
-
-
-
1
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
1
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392177
Ruvinov
Tetrameric N5-(L-1-carboxyethy ...
Lactococcus lactis
Arch. Biochem. Biophys.
371
115-123
1999
-
1
-
-
-
1
-
-
-
-
13
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
13
-
-
-
-
1
-
-
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
392178
Sackett
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
Protein Sci.
8
2121-2129
1999
-
1
1
1
-
-
-
-
-
-
4
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392176
Donkersloot
Cloning, expression, sequence ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
270
12226-12234
1995
-
2
1
-
-
-
-
-
-
-
1
-
-
7
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392175
Miller
Enzymatic synthesis and charac ...
Lactococcus lactis
Amino Acids
6
189-198
1994
-
1
1
-
-
-
2
1
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
1
1
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392174
Thompson
Transposon-encoded sucrose met ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
266
14573-14579
1991
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392173
Donkersloot
Simultaneous loss of N5-(carbo ...
Lactococcus lactis
J. Bacteriol.
172
4122-4126
1990
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392172
Thompson
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
J. Biol. Chem.
264
9592-9601
1989
-
-
-
-
-
1
1
4
-
-
5
-
-
1
-
-
1
-
-
-
1
1
3
2
-
1
2
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
-
-
5
-
-
-
-
1
-
-
1
1
3
2
-
1
2
-
1
1
-
-
-
-
-
-
-
-