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Literature summary for 1.5.1.24 extracted from

  • Thompson, J.
    N5-(L-1-carboxyethyl)-L-ornithine:NADP+ oxidoreductase from Streptococcus lactis. Purification and partial characterization (1989), J. Biol. Chem., 264, 9592-9601.
    View publication on PubMed

General Stability

General Stability Organism
addition of 20% glycerol stabilizes the enzyme Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
N5-(L-1-carboxyethyl)-L-ornithine
-
Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
NADPH
-
Lactococcus lactis
0.15
-
pyruvate
-
Lactococcus lactis
3.3
-
ornithine
-
Lactococcus lactis
18.2
-
lysine
-
Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000 38000 immunoblot analysis Lactococcus lactis
38000
-
2 * 38000, SDS-PAGE Lactococcus lactis
38000
-
4 * 38000, SDS-PAGE Lactococcus lactis
39200
-
calculated from amino acid sequence Lactococcus lactis
78000
-
gel filtration Lactococcus lactis
150000
-
PAGE, hight pressure liquid chromatography, gel filtration Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
subsp. lactis K1, formerly Streptococcus lactis K1
-
Lactococcus lactis
-
strains S.lactis K1 and 133
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.5
-
-
Lactococcus lactis

Storage Stability

Storage Stability Organism
-20°C, no significant loss of activity detected after 2 months of storage Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + pyruvate + NADPH pyruvic acid serves as an effective keto substrate for the condensation reaction Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
ir
lysine + pyruvate + NADPH
-
Lactococcus lactis N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
ir
ornithine + 3-fluoropyruvate + NADPH slight activity observed, about 15% of the rate found with pyruvate Lactococcus lactis N6-(1-carboxy-2-fluoroethyl)ornithine + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Lactococcus lactis
tetramer 4 * 38000, SDS-PAGE Lactococcus lactis

Synonyms

Synonyms Comment Organism
N5-CEO synthase
-
Lactococcus lactis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50
-
Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable to heating for 10 min Lactococcus lactis
60
-
rapidly inactivated by heating at Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Lactococcus lactis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
NADPH high affinity for NADPH, NADH cannot replace NADPH, but nicotinamide hypoxanthine dinucleotide phosphate, reduced form, is an effective substitute for the natural cofactor Lactococcus lactis