Application | Comment | Organism |
---|---|---|
medicine | deficiency and thermolability of enzyme as cause of mild homocysteinemia with premature vascular disease | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
5-methyltetrahydrofolate | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + reduced acceptor | Homo sapiens | homocysteine metabolism | 5-methyltetrahydrofolate + oxidized acceptor | 5-methyltetrahydrofolate is the main form of circulating folate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | Homo sapiens | physiological direction is the 5-methyltetrahydrofolate formation | 5-methyltetrahydrofolate + oxidized acceptor | 5-methyltetrahydrofolate is the main form of circulating folate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | Homo sapiens | remethylation of homocysteine to methionine | 5-methyltetrahydrofolate + oxidized acceptor | 5-methyltetrahydrofolate is the main form of circulating folate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
values of healthy and homocysteinemic humans before and after heat inactivation, specific activity of thermolabile variant of enzyme in homozygotes is 50% of normal enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Homo sapiens | 5-methyltetrahydrofolate + oxidized acceptor | - |
? | |
5,10-methylenetetrahydrofolate + reduced acceptor | homocysteine metabolism | Homo sapiens | 5-methyltetrahydrofolate + oxidized acceptor | 5-methyltetrahydrofolate is the main form of circulating folate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | physiological direction is the 5-methyltetrahydrofolate formation | Homo sapiens | 5-methyltetrahydrofolate + oxidized acceptor | 5-methyltetrahydrofolate is the main form of circulating folate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | remethylation of homocysteine to methionine | Homo sapiens | 5-methyltetrahydrofolate + oxidized acceptor | 5-methyltetrahydrofolate is the main form of circulating folate | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of enzyme from healthy and homocysteinemic humans after heat inactivation, the latter with a more thermolabile enzyme: residual activity in homozygotes is lower than 30% compared with 50% in control subjects | Homo sapiens |
40 | - |
5 min, 17% loss of activity | Homo sapiens |
46 | - |
5 min, 53% loss of activity | Homo sapiens |
49 | - |
5 min, 70% loss of activity | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme activity depends on the concentration of its cofactor FAD: maximum activity at 0.05 mM FAD | Homo sapiens |