General Stability | Organism |
---|---|
FAD stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | vitamin B12 and methionine can repress enzyme biosynthesis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + FADH2 | Escherichia coli | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | 5-methyltetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + FADH2 | Escherichia coli 113-3 | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | 5-methyltetrahydrofolate + FAD | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli 113-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
about 100fold purification | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + FADH2 | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | Escherichia coli | 5-methyltetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + FADH2 | biosynthesis of 5-methyltetrahydrofolate, a donor of methyl groups of methionine | Escherichia coli 113-3 | 5-methyltetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + reduced acceptor | under anaerobic conditions and in absence of electron acceptors, the equilibrium lies far to the 5-methyltetrahydrofolate formation, inclusion of menadione or oxygen promotes the oxidation of 5-methyltetrahydrofolate | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | under anaerobic conditions and in absence of electron acceptors, the equilibrium lies far to the 5-methyltetrahydrofolate formation, inclusion of menadione or oxygen promotes the oxidation of 5-methyltetrahydrofolate | Escherichia coli 113-3 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Escherichia coli 113-3 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
additional information | no direct activity with pyridine nucleotides | Escherichia coli | ? | - |
? | |
additional information | no direct activity with pyridine nucleotides | Escherichia coli 113-3 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | 6.4 | formation of 5-methyltetrahydrofolate | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme-bound FAD | Escherichia coli | |
FAD | flavoprotein, oxidized acceptor in reverse reaction | Escherichia coli | |
FADH2 | flavoprotein, reduced acceptor in forward reaction | Escherichia coli | |
additional information | no direct activity with pyridine nucleotides: NADH, NADPH | Escherichia coli |