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Literature summary for 1.5.1.20 extracted from
- 5,10-Methylenetetrahydrofolate reductase (MTHFR) assay in the forward direction: residual activity in MTHFR deficiency (2002), Clin. Chem., 48, 835-843.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C667T | high level of enzyme activity, loses almost all its activity after 20 min at 55°C, enzyme in patients with TT or TC genotype are more thermolabile than wild type enzyme at 46°C | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
5,10-methylenetetrahydrofolate | - |
Homo sapiens |  |
| 0.03 | - |
NADPH | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
patients with enzyme deficiency | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skin | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity in various patients with enzyme deficiencies | Homo sapiens |
| 0.413 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -75°C, stable for at least 9 months in cell pellets and enzyme extracts | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 6.9 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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