Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A177V | mutation does not affect Km or kcat values for NADH or 5,10-methylenetetrahydrofolate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
0.017 | - |
NADH | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
108000 | - |
A177V mutant enzyme, gel filtration | Escherichia coli |
190000 | - |
wild type enzyme, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEZ1 | - |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
tetramer | gel filtration | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.5 | - |
NADH | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli |