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Literature summary for 1.5.1.20 extracted from

  • Kutzbach, C.; Stokstad, E.L.R.
    Mammalian methylenetetrahydrofolate reductase. Partial purification, properties, and inhibition by S-adenosylmethionine (1971), Biochim. Biophys. Acta, 250, 459-477.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dicoumarol inhibits menadione reductase activity only Sus scrofa
Mersalyl 80% inhibition at 1 mM Sus scrofa
NADH substrate inhibition above 1 mM Sus scrofa
p-chloromercuribenzoate 60% inhibition of the methylenetetrahydrofolate reductase activity at 0.04 mM, 83% inhibition of the menadione reductase activity at 0.04 mM Sus scrofa
S-adenosylmethionine allosteric inhibition; inhibition partially reversed by S-adenosylhomocysteine Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
5,10-methylenetetrahydrofolate methylenetetrahydrofolate reductase reaction Sus scrofa
0.033
-
NADPH methylenetetrahydrofolate reductase reaction Sus scrofa
0.049
-
NADPH reduction of menadione Sus scrofa
0.085
-
NADH reduction of menadione Sus scrofa
0.31
-
NADH methylenetetrahydrofolate reductase reaction Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no evidence for participation of heavy metals ions in catalytic process Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH Rattus norvegicus
-
?
-
?
5,10-methylenetetrahydrofolate + NADPH Sus scrofa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
78
-
-
Rattus norvegicus
153
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADH + H+
-
Sus scrofa 5-methyltetrahydrofolate + NAD+
-
r
5,10-methylenetetrahydrofolate + NADPH ferricyanide, dichlorophenolindophenol and cytochrome c can act as electron acceptors Sus scrofa 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH equilibrium far on the side of methyltetrahydrofolate formation Rattus norvegicus 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH equilibrium far on the side of methyltetrahydrofolate formation Sus scrofa 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH specific for (+)-diastereoisomer Rattus norvegicus 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH specific for (+)-diastereoisomer Sus scrofa 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH
-
Rattus norvegicus ?
-
?
5,10-methylenetetrahydrofolate + NADPH
-
Sus scrofa ?
-
?
5-methyltetrahydrofolate + oxidized menadione
-
Rattus norvegicus 5,10-methylenetetrahydrofolate + reduced menadione
-
?
5-methyltetrahydrofolate + oxidized menadione
-
Sus scrofa 5,10-methylenetetrahydrofolate + reduced menadione
-
?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 methylenetetrahydrofolate reductase reaction, slow increase of activity between 25°C and 45°C Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
inactivation above Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 6.7 methylenetetrahydrofolate reductase reaction Sus scrofa
7.1
-
menadione reductase reaction Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8.6 about 60% of activity maximum at pH 5.8, about 50% of activity maximum at pH 8.6, menadione reductase activity Sus scrofa
6.2 7.3 half-maximal activity at pH 6.2 and pH 7.3, methylenetetrahydrofolate reductase reaction Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Sus scrofa
FMN no effect Rattus norvegicus
FMN no effect Sus scrofa
NADH low activity Sus scrofa
NADPH specific for Sus scrofa