Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dicoumarol | inhibits menadione reductase activity only | Sus scrofa | |
Mersalyl | 80% inhibition at 1 mM | Sus scrofa | |
NADH | substrate inhibition above 1 mM | Sus scrofa | |
p-chloromercuribenzoate | 60% inhibition of the methylenetetrahydrofolate reductase activity at 0.04 mM, 83% inhibition of the menadione reductase activity at 0.04 mM | Sus scrofa | |
S-adenosylmethionine | allosteric inhibition; inhibition partially reversed by S-adenosylhomocysteine | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
5,10-methylenetetrahydrofolate | methylenetetrahydrofolate reductase reaction | Sus scrofa | |
0.033 | - |
NADPH | methylenetetrahydrofolate reductase reaction | Sus scrofa | |
0.049 | - |
NADPH | reduction of menadione | Sus scrofa | |
0.085 | - |
NADH | reduction of menadione | Sus scrofa | |
0.31 | - |
NADH | methylenetetrahydrofolate reductase reaction | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no evidence for participation of heavy metals ions in catalytic process | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADPH | Rattus norvegicus | - |
? | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | Sus scrofa | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
78 | - |
- |
Rattus norvegicus |
153 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Sus scrofa | 5-methyltetrahydrofolate + NAD+ | - |
r | |
5,10-methylenetetrahydrofolate + NADPH | ferricyanide, dichlorophenolindophenol and cytochrome c can act as electron acceptors | Sus scrofa | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | equilibrium far on the side of methyltetrahydrofolate formation | Rattus norvegicus | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | equilibrium far on the side of methyltetrahydrofolate formation | Sus scrofa | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | specific for (+)-diastereoisomer | Rattus norvegicus | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | specific for (+)-diastereoisomer | Sus scrofa | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | - |
Rattus norvegicus | ? | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | - |
Sus scrofa | ? | - |
? | |
5-methyltetrahydrofolate + oxidized menadione | - |
Rattus norvegicus | 5,10-methylenetetrahydrofolate + reduced menadione | - |
? | |
5-methyltetrahydrofolate + oxidized menadione | - |
Sus scrofa | 5,10-methylenetetrahydrofolate + reduced menadione | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | methylenetetrahydrofolate reductase reaction, slow increase of activity between 25°C and 45°C | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
inactivation above | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 6.7 | methylenetetrahydrofolate reductase reaction | Sus scrofa |
7.1 | - |
menadione reductase reaction | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.6 | about 60% of activity maximum at pH 5.8, about 50% of activity maximum at pH 8.6, menadione reductase activity | Sus scrofa |
6.2 | 7.3 | half-maximal activity at pH 6.2 and pH 7.3, methylenetetrahydrofolate reductase reaction | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Sus scrofa | |
FMN | no effect | Rattus norvegicus | |
FMN | no effect | Sus scrofa | |
NADH | low activity | Sus scrofa | |
NADPH | specific for | Sus scrofa |