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Literature summary for 1.5.1.2 extracted from

  • Ruszkowski, M.; Nocek, B.; Forlani, G.; Dauter, Z.
    The structure of Medicago truncatula delta(1)-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants (2015), Front. Plant Sci., 6, 869.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Cl- progressive stimulation by chloride ions from 20-200 mM, inhibitory above. At 100 mM, 1.8fold stimulation in presence of NADH Medicago truncatula

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Medicago truncatula

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of unliganded P5CR decamer, and its complexes with the products NAD+, NADP+, and L-proline, to 1.7, 1.85, 1.95, and 2.1 A resolution, respectively Medicago truncatula

Inhibitors

Inhibitors Comment Organism Structure
Cl- progressive stimulation from 20-200 mM, inhibitory above Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
1-pyrroline-5-carboxylate cosubstrate NADPH, pH 7.5, 35°C Medicago truncatula
0.039
-
NADPH pH 7.5, 35°C Medicago truncatula
0.24
-
1-pyrroline-5-carboxylate cosubstrate NADH, pH 7.5, 35°C Medicago truncatula
0.468
-
NADH pH 7.5, 35°C Medicago truncatula

Organism

Organism UniProt Comment Textmining
Medicago truncatula G7KRM5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NADH + H+
-
Medicago truncatula L-proline + NAD+
-
?
1-pyrroline-5-carboxylate + NADPH + H+
-
Medicago truncatula L-proline + NADP+
-
?

Subunits

Subunits Comment Organism
More enzyme is a decamer of five dimers, crystallization data Medicago truncatula

Synonyms

Synonyms Comment Organism
MTR_7g090160
-
Medicago truncatula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.113
-
NADPH pH 7.5, 35°C Medicago truncatula
2.972
-
NADH pH 7.5, 35°C Medicago truncatula

Cofactor

Cofactor Comment Organism Structure
NADH
-
Medicago truncatula
NADPH
-
Medicago truncatula

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.485
-
cofactor NADH, pH 7.5, 35°C Medicago truncatula Cl-
0.704
-
cofactor NADPH, pH 7.5, 35°C Medicago truncatula Cl-

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6350
-
NADH pH 7.5, 35°C Medicago truncatula
28500
-
NADPH pH 7.5, 35°C Medicago truncatula