Application | Comment | Organism |
---|---|---|
medicine | pyrroline-5-carboxylate reductase is related to virulence of Mycobacterium tuberculosis | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
into the pET30a vector for expression in Escherichia coli BL21DE3 | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | - |
Mycobacterium tuberculosis | |
H2O2 | - |
Mycobacterium tuberculosis | |
methanol | - |
Mycobacterium tuberculosis | |
SDS | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
1-pyrroline-5-carboxylate | cofactor NADH | Mycobacterium tuberculosis | |
0.047 | - |
1-pyrroline-5-carboxylate | cofactor NADPH | Mycobacterium tuberculosis | |
0.057 | - |
NADH | - |
Mycobacterium tuberculosis | |
0.059 | - |
NADPH | - |
Mycobacterium tuberculosis | |
0.093 | - |
NADP+ | - |
Mycobacterium tuberculosis | |
0.12 | - |
L-proline | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity is significantly increased in the forward reaction, optimal concentration of the cation is about 50-100 microM | Mycobacterium tuberculosis | |
Mn2+ | activity is significantly increased in the forward reaction, optimal concentration of the cation is about 50-100 microM | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
after enterokinase treatment, estimated by SDS-PAGE | Mycobacterium tuberculosis |
365000 | - |
decamer, analysis of nondegenerated protein by native gel electrophoresis | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NADPH + H+ | Mycobacterium tuberculosis | - |
L-proline + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA His-binding resin affinity chromatography, the N-terminal fusion partner is cleaved by enterokinase | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
purification step supernatant, reverse reaction | Mycobacterium tuberculosis |
1.7 | - |
purification step supernatant, forward reaction | Mycobacterium tuberculosis |
30 | - |
purification step Ni-NTA column, reverse reaction | Mycobacterium tuberculosis |
57 | - |
purification step Ni-NTA column, forward reaction | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NADH + H+ | - |
Mycobacterium tuberculosis | L-proline + NAD+ | - |
r | |
1-pyrroline-5-carboxylate + NADPH + H+ | - |
Mycobacterium tuberculosis | L-proline + NADP+ | - |
r | |
1-pyrroline-5-carboxylate + NADPH + H+ | activity assay, forward reaction | Mycobacterium tuberculosis | L-proline + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
decamer | 10*32000-35600, estimated by SDS-PAGE, confirmed by spectrometric analysis | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
P5CR | - |
Mycobacterium tuberculosis |
pyrroline-5-carboxylate reductase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay, forward and reverse reaction | Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half life of the His-tagged protein is about 20 min | Mycobacterium tuberculosis |
70 | - |
His-tagged protein is rapidly inactivated | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00983 | - |
1-pyrroline-5-carboxylate | cofactor NADPH | Mycobacterium tuberculosis | |
137.9 | - |
NADP+ | - |
Mycobacterium tuberculosis | |
212.8 | - |
L-proline | - |
Mycobacterium tuberculosis | |
310.2 | - |
NADH | - |
Mycobacterium tuberculosis | |
321.6 | - |
NADPH | - |
Mycobacterium tuberculosis | |
387.3 | - |
1-pyrroline-5-carboxylate | cofactor NADH | Mycobacterium tuberculosis | |
451.7 | - |
1-pyrroline-5-carboxylate | cofactor NADPH | Mycobacterium tuberculosis | |
4000 | - |
NADPH | - |
Mycobacterium tuberculosis | |
4170 | - |
NADH | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay, forward reaction | Mycobacterium tuberculosis |
10.3 | - |
activity assay, reverse reaction | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |