Literature summary for 1.5.1.2 extracted from

Meng, Z.; Lou, Z.; Liu, Z.; Li, M.; Zhao, X.; Bartlam, M.; Rao, Z.
Crystal structure of human pyrroline-5-carboxylate reductase (2006), J. Mol. Biol., 359, 1364-1377.

Search for more literature for 1.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
cloned into the pET28a+ vector with a His6 tag at the N-terminus for expression in the Escherichia coli strain B834DE3	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
2.8 A resolution structure of the pyrroline-5-carboxylate reductase apo enzyme, and its ternary complex with NADPH and substrate analog at 3.1 A	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E221A	affinity for substrates is increased	Homo sapiens
E221G	insoluble protein	Homo sapiens
additional information	N-truncation results in an insoluble protein, C-truncation does not alter the activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
proline	competitive inhibitor of P5CR NAD(P)H-dependent thioproline dehydrogenase activity	Homo sapiens
stearoyl-CoA	competitive inhibitor of P5CR NAD(P)H-dependent thioproline dehydrogenase activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.151	-	NAD+	wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay	Homo sapiens
0.235	-	NAD+	E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay	Homo sapiens
0.48	-	NADP+	E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay	Homo sapiens
0.73	-	NADP+	E221A mutant, fixed 1 mM NADP+	Homo sapiens
1.26	-	NADP+	wild type enzyme, fixed 1 mM NADP+	Homo sapiens
3.06	-	NADP+	wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-proline + NAD(P)+	Homo sapiens	-	1-pyrroline-5-carboxylate + NAD(P)H + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a Ni-NTA agarose, a Superdex200 and a Resource Q column	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
refolding after treatment with urea	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-proline + NAD(P)+	-	Homo sapiens	1-pyrroline-5-carboxylate + NAD(P)H + H+	-	r

Subunits

Subunits	Comment	Organism
decamer	five homodimer subunits	Homo sapiens

Synonyms

Synonyms	Comment	Organism
P5CR	-	Homo sapiens
pyrroline-5-carboxylate reductase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	activity assay	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
13	-	NADP+	E221A mutant, fixed 1 mM NADP+	Homo sapiens
55	-	NADP+	wild type enzyme, fixed 1 mM NADP+	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	activity assay	Homo sapiens
10	-	L-proline dehydrogenase activity assay	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Homo sapiens
NADH	-	Homo sapiens
NADP+	-	Homo sapiens
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)