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Literature summary for 1.5.1.2 extracted from
- Pyrroline-5-carboxylate reductase from cucurbita cotyledons (1973), Phytochemistry, 12, 1565-1568.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | stimulation of undialyzed enzyme at 30 mM | Cucurbita maxima |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 65% inactivation at 0.3 mM | Cucurbita maxima | |
| ATP | 41% inactivation at 1 mM | Cucurbita maxima | |
| ATP | - |
Cucurbita moschata | |
| Hg2+ | 65% inactivation at 0.3 mM | Cucurbita maxima | |
| Hg2+ | - |
Cucurbita moschata | |
| NADP+ | 43% inactivation at 1 mM | Cucurbita maxima | |
| NH2OH | 92% inactivation at 1 mM | Cucurbita maxima | |
| NH2OH | - |
Cucurbita moschata | |
| p-chloromercuribenzoate | not | Cucurbita maxima | |
| p-chloromercuribenzoate | not | Cucurbita moschata | |
| proline | 12% inactivation at 50 mM | Cucurbita maxima | |
| proline | - |
Cucurbita moschata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
NADH | - |
Cucurbita moschata | |
| 0.09 | - |
1-pyrroline-5-carboxylate | - |
Cucurbita moschata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Cucurbita maxima | - |
- |
| soluble | - |
Cucurbita moschata | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucurbita maxima | - |
- |
- |
| Cucurbita moschata | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Cucurbita maxima | - |
| cotyledon | - |
Cucurbita moschata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.08 | - |
- |
Cucurbita maxima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
Cucurbita moschata | L-proline + NAD(P)+ | - |
? | |
| 1-pyrroline-5-carboxylate + NAD(P)H + H+ | reaction is irreversible at pH 7.4, but reversible at pH 10.3 | Cucurbita maxima | L-proline + NAD(P)+ | - |
r | |
| 1-pyrroline-5-carboxylate + NADH + H+ | - |
Cucurbita moschata | L-proline + NAD+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.6 | - |
Cucurbita maxima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | preferentially | Cucurbita maxima | |
| NADH | preferentially | Cucurbita moschata | |
| NADPH | - |
Cucurbita maxima | |
| NADPH | - |
Cucurbita moschata | |
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Release 2023.1 (January 2023)
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