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Literature summary for 1.5.1.2 extracted from

  • Laliberte, G.; Hellebust, J.A.
    Pyrroline-5-carboxylate reductase in Chlorella atuotrophica and chlorella saccharophila in relation to osmoregulation (1989), Plant Physiol., 91, 917-923.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.2

General Stability

General Stability Organism
sorbitol stabilizes Chlorella vulgaris var. autotrophica
sorbitol stabilizes Chloroidium saccharophilum
thiol reagents stabilize Chlorella vulgaris var. autotrophica
thiol reagents stabilize Chloroidium saccharophilum

Inhibitors

Inhibitors Comment Organism Structure
KCl 68% inhibition at 2 M Chlorella vulgaris var. autotrophica
KCl
-
 Chloroidium saccharophilum
NaCl 70% inhibition at 2 M Chlorella vulgaris var. autotrophica
NaCl
-
 Chloroidium saccharophilum
NADH substrate inhibition Chlorella vulgaris var. autotrophica
NADH substrate inhibition Chloroidium saccharophilum
proline 89% inhibition at 2 M Chlorella vulgaris var. autotrophica
proline
-
 Chloroidium saccharophilum
pyrroline-5-carboxylate substrate inhibition Chlorella vulgaris var. autotrophica
pyrroline-5-carboxylate substrate inhibition Chloroidium saccharophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 NADH
-
 Chlorella vulgaris var. autotrophica
0.1
-
 NADH
-
 Chloroidium saccharophilum
0.17
-
 1-pyrroline-5-carboxylate
-
 Chlorella vulgaris var. autotrophica
0.17
-
 1-pyrroline-5-carboxylate
-
 Chloroidium saccharophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyrroline-5-carboxylate + NAD(P)H + H+ Chlorella vulgaris var. autotrophica discussion of role in vivo L-proline + NAD(P)+
-
 ?
pyrroline-5-carboxylate + NAD(P)H + H+ Chloroidium saccharophilum discussion of role in vivo L-proline + NAD(P)+
-
 ?
pyrroline-5-carboxylate + NAD(P)H + H+ Chlorella vulgaris var. autotrophica ping pong mechanism L-proline + NAD(P)+
-
 ?
pyrroline-5-carboxylate + NAD(P)H + H+ Chloroidium saccharophilum ping pong mechanism L-proline + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorella vulgaris var. autotrophica
-
-
-
Chloroidium saccharophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Chlorella vulgaris var. autotrophica
partial Chloroidium saccharophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Chlorella vulgaris var. autotrophica L-proline + NAD(P)+
-
 ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Chloroidium saccharophilum L-proline + NAD(P)+
-
 ir
1-pyrroline-5-carboxylate + NADH + H+
-
 Chlorella vulgaris var. autotrophica L-proline + NAD+
-
 r
1-pyrroline-5-carboxylate + NADH + H+
-
 Chloroidium saccharophilum L-proline + NAD+
-
 r
pyrroline-5-carboxylate + NAD(P)H + H+ discussion of role in vivo Chlorella vulgaris var. autotrophica L-proline + NAD(P)+
-
 ?
pyrroline-5-carboxylate + NAD(P)H + H+ discussion of role in vivo Chloroidium saccharophilum L-proline + NAD(P)+
-
 ?
pyrroline-5-carboxylate + NAD(P)H + H+ ping pong mechanism Chlorella vulgaris var. autotrophica L-proline + NAD(P)+
-
 ?
pyrroline-5-carboxylate + NAD(P)H + H+ ping pong mechanism Chloroidium saccharophilum L-proline + NAD(P)+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Chlorella vulgaris var. autotrophica
7 8
-
 Chloroidium saccharophilum

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 less than 50% of maximal activity above and below Chlorella vulgaris var. autotrophica
6 8.5 less than 50% of maximal activity above and below Chloroidium saccharophilum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2
-
 best value for stability Chlorella vulgaris var. autotrophica
7.2
-
 best value for stability Chloroidium saccharophilum

Cofactor

Cofactor Comment Organism Structure
NADH specific for Chlorella vulgaris var. autotrophica
NADH specific for Chloroidium saccharophilum