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Literature summary for 1.5.1.19 extracted from

  • Turgut, A.; Basim, H.
    Development of a real-time PCR assay using locked nucleic acid probe for the detection of octopine, nopaline, and vitopine strains of tumorigenic Allorhizobium vitis in grapevines (2021), Crop Prot., 145, 105620 .
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Cloned(Commentary)

Cloned (Comment) Organism
gene GN325_22415 or nos, cloning from strain ICMP 10753, real-time PCR enzyme expression analysis Agrobacterium vitis

Protein Variants

Protein Variants Comment Organism
additional information development of a real-time PCR assay using locked nucleic acid probe for the detection of octopine, nopaline, and vitopine strains of tumorigenic Allorhizobium vitis in grapevines Agrobacterium vitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O Agrobacterium vitis
-
 L-arginine + 2-oxoglutarate + NADPH + H+
-
 r
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O Agrobacterium vitis ICMP 10753
-
 L-arginine + 2-oxoglutarate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Agrobacterium vitis A0A1S2E988
-
-
Agrobacterium vitis ICMP 10753 A0A1S2E988
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
-
 Agrobacterium vitis L-arginine + 2-oxoglutarate + NADPH + H+
-
 r
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
-
 Agrobacterium vitis ICMP 10753 L-arginine + 2-oxoglutarate + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
GN325_22415
-
 Agrobacterium vitis
nopaline synthase
-
 Agrobacterium vitis
NOS
-
 Agrobacterium vitis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Agrobacterium vitis

General Information

General Information Comment Organism
additional information crown gall disease caused by Allorhizobium vitis is one of the most destructive diseases in grapevine cultivation from an economical perspective. The bacterial pathogen exists systemically in grapevines and spreads through cuttings used for propagation material obtained from grapevines, living vines, and mother blocks, which poses a high risk for certified grapevine nursery material cultivation, nursery industry, and growers. Therefore, in this study, real-time PCR-based methods were developed for effectively and accurately determining the presence and the density of the bacterial pathogen in grapevine nursery material and the data used for indexing programs. Octopine-, nopaline-, and vitopine-catabolizing A. vitis strain-specific primer and locked nucleic acid (LNA) probe sets were determined depending on the ocs, nos, vis, vitopine iaaM, and vitopine virD2 genes. Primer-probe sets were then used to amplify the octopine synthase gene (62 bp) from octopine strains, the nopaline synthase gene (78 bp) from nopaline strains, and the vitopine synthase gene (60 bp) from vitopine strains, as well as the vitopine iaaM gene (60 bp) and the vitopine virD2 gene (66 bp) of A. vitis. The detection of Allorhizobium vitis strains from bacterial suspension or extracted plant sap can be achieved within a short time (i.e. 20-25 min) with real-time PCR-based assays using the designed primer and probe sets, method development and evaluation, overview Agrobacterium vitis