Cloned (Comment) | Organism |
---|---|
heterologously overproduced in Escherichia coli with a C-terminal His6-tag | Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
5,10-methylenetetrahydrofolate | pH 6.0, 37°C, the Km-value for 5,10-methylenetetrahydrofolate in the presence of 2.7 mM NAD+ is below 0.005 mM | Methanosarcina barkeri | |
0.15 | - |
NAD+ | pH 6.0, 37°C | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Methanosarcina barkeri |
318700 | - |
x * 318700, calculated from sequence | Methanosarcina barkeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | Q46A53 | - |
- |
Methanosarcina barkeri DSM 804 | Q46A53 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | 2 | pH 6.0, 37°C, purified enzyme, recombinant | Methanosarcina barkeri |
5 | - |
pH 6.0, 37°C, extract from Escherichia coli cells carrying the expression vector | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NAD+ | the bifunctional enzyme also shows N5,N10-methenyltetrahydrofolate cyclohydrolase activity EC 3.5.4.9. with NADP+ (1 mM), the enzyme shows less than 0.1% of the activity obtained with NAD+ (1 mM). The purified enzyme shows no activity with methylene-tetrahydromethanopterin or with 5,10-methylenetetrahydrosarcinapterin either in the presence of NAD+ or NADP+ or in the presence of F420 | Methanosarcina barkeri | 5,10-methenyltetrahydrofolate + NADH + H+ | - |
? | |
5,10-methylenetetrahydrofolate + NAD+ | the bifunctional enzyme also shows N5,N10-methenyltetrahydrofolate cyclohydrolase activity EC 3.5.4.9. with NADP+ (1 mM), the enzyme shows less than 0.1% of the activity obtained with NAD+ (1 mM). The purified enzyme shows no activity with methylene-tetrahydromethanopterin or with 5,10-methylenetetrahydrosarcinapterin either in the presence of NAD+ or NADP+ or in the presence of F420 | Methanosarcina barkeri DSM 804 | 5,10-methenyltetrahydrofolate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Methanosarcina barkeri |
? | x * 318700, calculated from sequence | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase | bifunctional enzyme EC 1.5.1.15/EC 3.5.4.9 | Methanosarcina barkeri |
FolD | bifunctional enzyme EC 1.5.1.15/EC 3.5.4.9 | Methanosarcina barkeri |
NAD+-dependent methylene-H4F dehydrogenase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | with NADP+ (1 mM), the enzyme shows less than 0.1% of the activity obtained with NAD+ (1 mM) | Methanosarcina barkeri |