Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Mus musculus | |
phosphate | absolute requirement, phosphate can be replaced by its analog arsenate | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloned cDNA for the human enzyme expressed in Escherichia coli K-38 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
L-5,10-methylenetetrahydrofolate | - |
Homo sapiens | |
0.013 | - |
NAD+ | - |
Spodoptera frugiperda | |
0.019 | - |
L-5,10-methylenetetrahydrofolate | - |
Mus musculus | |
0.03 | - |
methenyltetrahydrofolate | - |
Spodoptera frugiperda | |
0.096 | - |
methenyltetrahydrofolate | - |
Homo sapiens | |
0.111 | - |
NAD+ | - |
Mus musculus | |
0.132 | - |
NAD+ | - |
Homo sapiens | |
0.155 | - |
5,10-methenyltetrahydrofolate | - |
Mus musculus | |
0.17 | - |
phosphate | - |
Mus musculus | |
0.19 | - |
phosphate | - |
Homo sapiens | |
0.25 | - |
Mg2+ | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Drosophila melanogaster | 5737 | - |
cytoplasm | - |
Mus musculus | 5737 | - |
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
cytoplasm | - |
Spodoptera frugiperda | 5737 | - |
mitochondrion | - |
Drosophila melanogaster | 5739 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus | |
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
- |
Mus musculus |
34000 | - |
- |
Homo sapiens |
37000 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-5,10-methenyl-tetrahydrofolate + NADH | Drosophila melanogaster | - |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | Mus musculus | - |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | Homo sapiens | - |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | Saccharomyces cerevisiae | - |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | Spodoptera frugiperda | - |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | Drosophila melanogaster | - |
5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | Mus musculus | - |
5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | Homo sapiens | - |
5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | Saccharomyces cerevisiae | - |
5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | Spodoptera frugiperda | - |
5,10-methenyl-tetrahydrofolate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
mouse, Ehrlich ascites tumor cells | - |
Saccharomyces cerevisiae | - |
- |
- |
Spodoptera frugiperda | - |
fall armyworm, cell line Sf9 | - |
Purification (Comment) | Organism |
---|---|
- |
Spodoptera frugiperda |
from ascites tumor cells | Mus musculus |
recombinant enzyme, expressed in Escherichia coli | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.9 | - |
- |
Mus musculus |
40 | 44 | - |
Homo sapiens |
43 | - |
- |
Spodoptera frugiperda |
Storage Stability | Organism |
---|---|
-80°C, enzyme is stable in 20-30% glycerol buffer and can be frozen for months without loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-5,10-methenyl-tetrahydrofolate + NADH | - |
Drosophila melanogaster | L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | - |
Mus musculus | L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | - |
Homo sapiens | L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | - |
Saccharomyces cerevisiae | L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methenyl-tetrahydrofolate + NADH | - |
Spodoptera frugiperda | L-5,10-methylenetetrahydrofolate + NAD+ | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
Drosophila melanogaster | 5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
Mus musculus | 5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
Homo sapiens | 5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
Saccharomyces cerevisiae | 5,10-methenyl-tetrahydrofolate + NADH | - |
r | |
L-5,10-methylenetetrahydrofolate + NAD+ | - |
Spodoptera frugiperda | 5,10-methenyl-tetrahydrofolate + NADH | - |
r |