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Literature summary for 1.5.1.15 extracted from

  • Smith, G.K.; Banks, S.D.; Monaco, T.J.; Rigual, R.; Duch, D.S.; Mullin, R.J.; Huber, B.E.
    Activity of an NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase in normal tissue, neoplastic cells, and oncogene-transformed cells (1990), Arch. Biochem. Biophys., 283, 367-371.
    View publication on PubMed
Search for more literature for 1.5.1.15

Application

Application Comment Organism
medicine
-
 Mus musculus
medicine NAD+ dependent isoenzyme is genuinely tumor-specific, it may provide a novel therapeutic target for cancer therapy and/or an important diagnostic tool Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.171
-
 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Homo sapiens
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Rattus norvegicus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Bos taurus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Rattus norvegicus Fischer
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Homo sapiens
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Rattus norvegicus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Bos taurus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Rattus norvegicus Fischer
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-
Homo sapiens
-
-
-
Mus musculus
-
 Ehrlich ascites line Swiss mice
-
Rattus norvegicus
-
 Fischer
-
Rattus norvegicus
-
 rat, Sprague-Dawley
-
Rattus norvegicus Fischer
-
 Fischer
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Rattus norvegicus
-
adrenal cortex
-
 Bos taurus
-
adrenal medulla
-
 Rattus norvegicus
-
bone marrow
-
 Mus musculus
-
bone marrow
-
 Rattus norvegicus
-
epithelium
-
 Rattus norvegicus
-
fibroblast
-
 Mus musculus
-
fibroblast
-
 Homo sapiens
-
liver
-
 Mus musculus
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0001
-
 C-57/Bl, bone marrow and whole adrenal, CD-1, adrenal Mus musculus
0.0002
-
 adrenal cortex Bos taurus
0.0002
-
 CD-1, spleen Mus musculus
0.0003
-
 cell line MRC-5 Homo sapiens
0.0003
-
 HEP-G2 tumor in nude mice Homo sapiens
0.0003
-
 RLE cells and RLE cells virus J5 v-myc oncogene-transformed Rattus norvegicus
0.0004
-
 CD-1, bone marrow Mus musculus
0.0005
-
 colon 38 solid tumor Mus musculus
0.0005
-
 human liver tumor Homo sapiens
0.0006
-
 RLE cells, virus 3611.MSV v-raf oncogene-transformed Rattus norvegicus
0.0011
-
 Swiss, bone marrow Mus musculus
0.0012
-
 cell line Hl-60 Homo sapiens
0.0012
-
 RLE cells, spontaneous oncogene transformed Rattus norvegicus
0.0014
-
 adrenal medulla Rattus norvegicus
0.0015
-
 cell line HEP-2 Homo sapiens
0.0015
-
 RLE cells, aflatoxin oncogene-transformed Rattus norvegicus
0.0017
-
 cell line B-16 solid tumor Mus musculus
0.002
-
 RLE cells, virus pRNR16 v-Ha-ras oncogene-transformed Rattus norvegicus
0.0021
-
 RLE cells, virus J2 v-raf/v-myc oncogene-transformed Rattus norvegicus
0.0027
-
 cell line MCF-7, 100% confluent Homo sapiens
0.0028
-
 cell line KB Homo sapiens
0.0035
-
 cell line MCF-7, 50% confluent Homo sapiens
0.0039
-
 cell line P-388 in vitro Mus musculus
0.004
-
 cell line CCRF-CEM Homo sapiens
0.0042
-
 cell line Y1 Mus musculus
0.0045
-
 cell line CHO Mus musculus
0.005
-
 Ehrlich ascites Mus musculus
0.0052
-
 adrenal cortex Rattus norvegicus
0.0058
-
 whole adrenal Rattus norvegicus
0.0063
-
 cell line B-16 in vitro Mus musculus
0.0063
-
 cell line MOLT-4 Homo sapiens
0.0071
-
 cell line P-388 leukemia ip Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Homo sapiens L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Rattus norvegicus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Bos taurus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Rattus norvegicus Fischer L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Rattus norvegicus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Bos taurus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Rattus norvegicus Fischer 5,10-methenyl-tetrahydrofolate + NADH
-
 r
additional information
-
 Homo sapiens ?
-
 ?
additional information level of enzyme activity appears to be strain dependent with Swiss mice having the highest activity Mus musculus ?
-
 ?