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Literature summary for 1.5.1.15 extracted from

  • Ragsdale, S.W.; Ljungdahl, L.G.
    Purification and properties of NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Acetobacterium woodii (1984), J. Biol. Chem., 259, 3499-3503.
    View publication on PubMed
Search for more literature for 1.5.1.15

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Acetobacterium woodii

Inhibitors

Inhibitors Comment Organism Structure
Zn2+
-
 Acetobacterium woodii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 L-5,10-methylenetetrahydrofolate
-
 Acetobacterium woodii
1
-
 5,10-methenyltetrahydrofolate
-
 Acetobacterium woodii
2
-
 NADH
-
 Acetobacterium woodii
4
-
 NAD+
-
 Acetobacterium woodii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus
Mn2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26300
-
 2 * 26300, amino acid analysis Acetobacterium woodii
26900
-
 2 * 26900, SDS-PAGE Acetobacterium woodii
55000
-
 purified enzyme, gel filtration Acetobacterium woodii
58000
-
 gel filtration Acetobacterium woodii
69000
-
 gel filtration Acetobacterium woodii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Acetobacterium woodii physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Acetobacterium woodii
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
-
-
-
Clostridium formicaceticum
-
-
-
Mus musculus
-
 mouse, Ehrlich ascites tumor cells
-
Saccharomyces cerevisiae
-
 yeast
-

Oxidation Stability

Oxidation Stability Organism
oxygen-labile Acetobacterium woodii

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium formicaceticum
-
 Acetobacterium woodii

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
670
-
-
 Acetobacterium woodii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Acetobacterium woodii 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Mus musculus
dimer
-
 Saccharomyces cerevisiae
dimer
-
 Clostridium formicaceticum
dimer 2 * 26300, amino acid analysis Acetobacterium woodii
dimer 2 * 26900, SDS-PAGE Acetobacterium woodii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.58
-
 L-5,10-methenyltetrahydrofolate
-
 Acetobacterium woodii
1.63
-
 L-5,10-methylenetetrahydrofolate
-
 Acetobacterium woodii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mus musculus
NAD+
-
 Saccharomyces cerevisiae
NAD+
-
 Clostridium formicaceticum
NAD+
-
 Acetobacterium woodii