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Literature summary for 1.5.1.15 extracted from

  • Moore, M.R.; O'Brien, W.E.; Ljungdahl, L.G.
    Purification and characterisation of nicotinamide adenine dinucleotide-dependent methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum (1974), J. Biol. Chem., 249, 5250-5253.
    View publication on PubMed
Search for more literature for 1.5.1.15

Inhibitors

Inhibitors Comment Organism Structure
EDTA NAD+ dependent enzyme from Ehrlich ascites tumor cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 L-5,10-methylenetetrahydrofolate
-
 Clostridium formicaceticum
0.79
-
 NAD+
-
 Clostridium formicaceticum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus
Mn2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE Clostridium formicaceticum
60000
-
 sedimentation equilibrium centrifugation Clostridium formicaceticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Gottschalkia acidurici
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Gottschalkia acidurici
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Clostridium formicaceticum
-
 strain ATCC 23439
-
Gottschalkia acidurici
-
-
-
Mus musculus
-
 mouse, Ehrlich ascites tumor cells
-
Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae
-
 Clostridium formicaceticum

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1400
-
-
 Clostridium formicaceticum

Storage Stability

Storage Stability Organism
4°C, can be stored dissolved in 0.1 M potassium maleate, pH 7.0 for at least 1 month without significant loss of activity Clostridium formicaceticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Gottschalkia acidurici L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Gottschalkia acidurici 5,10-methenyl-tetrahydrofolate + NADH
-
 r
additional information
-
 Mus musculus ?
-
 ?
additional information
-
 Saccharomyces cerevisiae ?
-
 ?
additional information active only with L-5,10-methylenetetrahydrofolate, the L-isomer does not affect the reaction Clostridium formicaceticum ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Mus musculus
dimer
-
 Saccharomyces cerevisiae
dimer 2 * 32000, SDS-PAGE Clostridium formicaceticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 activity increases to the optimum, above 45°C the enzyme begins to denature Clostridium formicaceticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for 30 min at temperatures below, loses 25% of its activity during incubation Clostridium formicaceticum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharomyces cerevisiae
NAD+ active with both NAD+ and NADP+ Gottschalkia acidurici
NAD+ specific for NAD+, no activity with NADP+ Clostridium formicaceticum
NAD+ Ehrlich ascites tumor cells contains 2 enzymes, NAD+ dependent and NADP+ dependent dehydrogenase Mus musculus
NADP+ active with both NAD+ and NADP+ Gottschalkia acidurici