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Literature summary for 1.5.1.10 extracted from

  • Xi, Z.; Yang, K.; Sheng, J.; Zhang, X.; Guo, J.; Meng, D.
    Novel chimeric spermidine synthase-saccharopine dehydrogenase gene with a possible role in postharvest development of the button mushroom (Agaricus bisporus) (2020), J. Hortic. Sci. Biotechnol., 95, 712-721 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene nSpe-Sdh, encoding a chimeric bifunctional gene with a single transcript that also encodes saccharopine dehydrogenase (SDH), DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O Agaricus bisporus
-
L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O Agaricus bisporus Sylvan A15
-
L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Agaricus bisporus D7GL19 strain Sylvan A15 commercially cultured in China
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Agaricus bisporus Sylvan A15 D7GL19 strain Sylvan A15 commercially cultured in China
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to over 85% purity Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Agaricus bisporus
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fruit capsule
-
Agaricus bisporus
-
gill
-
Agaricus bisporus
-
additional information developmental stage and tissue specific expression analysis, overview Agaricus bisporus
-
stipe
-
Agaricus bisporus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
Agaricus bisporus L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
Agaricus bisporus Sylvan A15 L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+
-
r

Subunits

Subunits Comment Organism
? x * 83630, sequence calculation, x * 84000, recombinant His-tagged enzyme, SDS-PAGE Agaricus bisporus
More the nSpe-Sdh gene contains a Spermidine_synt_N InterPro domain (IPR035246) at its N-terminal region, existence of a linker region (approximately 60 nucleotides) between Spe and Sdh in Agaricus bisporus Agaricus bisporus

Synonyms

Synonyms Comment Organism
nSpe-Sdh
-
Agaricus bisporus
saccharopine dehydrogenase
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Agaricus bisporus
SDH
-
Agaricus bisporus
spermidine synthase-saccharopine dehydrogenase
-
Agaricus bisporus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Agaricus bisporus
NADPH
-
Agaricus bisporus

pI Value

Organism Comment pI Value Maximum pI Value
Agaricus bisporus sequence calculation
-
5.76

Expression

Organism Comment Expression
Agaricus bisporus once the protecting veil is broken during storage, the expression levels decrease to an initial storage level and remain relatively constant until the end of the storage, indicating the possible involvement of nSpe-Sdh in postharvest mushroom development down
Agaricus bisporus the expression of nSPE-SDH mRNA and protein are both significantly upregulated with the cap expansion of Agaricus bisporus during storage. The up-regulation of the expression of nSPE-SDH mRNA and protein was both tissue especially in cap tissues up

General Information

General Information Comment Organism
evolution after the appearance of the Spe-Sdh gene, the association of the Spe and Sdh genes remained throughout evolution, phylogenetic analysis. The linker region between Spe and Sdh (approximately 60 nucleotides) may have evolved specifically in Basidiomycota to regulate Basidiomycotaspecific processes Agaricus bisporus
physiological function enzyme nSpe-Sdh is a bifunctional, chimeric enzyme. SPESDH is involved in Agaricus bisporus postharvest development and is tissue-specially upregulated in cap tissues Agaricus bisporus