Cloned (Comment) | Organism |
---|---|
gene nSpe-Sdh, encoding a chimeric bifunctional gene with a single transcript that also encodes saccharopine dehydrogenase (SDH), DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | Agaricus bisporus | - |
L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+ | - |
r | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | Agaricus bisporus Sylvan A15 | - |
L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | D7GL19 | strain Sylvan A15 commercially cultured in China | - |
Agaricus bisporus Sylvan A15 | D7GL19 | strain Sylvan A15 commercially cultured in China | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to over 85% purity | Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Agaricus bisporus | - |
fruit capsule | - |
Agaricus bisporus | - |
gill | - |
Agaricus bisporus | - |
additional information | developmental stage and tissue specific expression analysis, overview | Agaricus bisporus | - |
stipe | - |
Agaricus bisporus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
Agaricus bisporus | L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+ | - |
r | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
Agaricus bisporus Sylvan A15 | L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 83630, sequence calculation, x * 84000, recombinant His-tagged enzyme, SDS-PAGE | Agaricus bisporus |
More | the nSpe-Sdh gene contains a Spermidine_synt_N InterPro domain (IPR035246) at its N-terminal region, existence of a linker region (approximately 60 nucleotides) between Spe and Sdh in Agaricus bisporus | Agaricus bisporus |
Synonyms | Comment | Organism |
---|---|---|
nSpe-Sdh | - |
Agaricus bisporus |
saccharopine dehydrogenase | - |
Agaricus bisporus |
SDH | - |
Agaricus bisporus |
spermidine synthase-saccharopine dehydrogenase | - |
Agaricus bisporus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Agaricus bisporus | |
NADPH | - |
Agaricus bisporus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Agaricus bisporus | sequence calculation | - |
5.76 |
Organism | Comment | Expression |
---|---|---|
Agaricus bisporus | once the protecting veil is broken during storage, the expression levels decrease to an initial storage level and remain relatively constant until the end of the storage, indicating the possible involvement of nSpe-Sdh in postharvest mushroom development | down |
Agaricus bisporus | the expression of nSPE-SDH mRNA and protein are both significantly upregulated with the cap expansion of Agaricus bisporus during storage. The up-regulation of the expression of nSPE-SDH mRNA and protein was both tissue especially in cap tissues | up |
General Information | Comment | Organism |
---|---|---|
evolution | after the appearance of the Spe-Sdh gene, the association of the Spe and Sdh genes remained throughout evolution, phylogenetic analysis. The linker region between Spe and Sdh (approximately 60 nucleotides) may have evolved specifically in Basidiomycota to regulate Basidiomycotaspecific processes | Agaricus bisporus |
physiological function | enzyme nSpe-Sdh is a bifunctional, chimeric enzyme. SPESDH is involved in Agaricus bisporus postharvest development and is tissue-specially upregulated in cap tissues | Agaricus bisporus |