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Literature summary for 1.5.1.10 extracted from

  • Naranjo, L.; Martin de Valmaseda, E.; Casqueiro, J.; Ullan, R.V.; Lamas-Maceiras, M.; Banuelos, O.; Martin, J.F.
    Inactivation of the lys7 gene, encoding saccharopine reductase in Penicillium chrysogenum, leads to accumulation of the secondary metabolite precursors piperideine-6-carboxylic acid and pipecolic acid from alpha-aminoadipic acid (2004), Appl. Environ. Microbiol., 70, 1031-1039.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene lys, subcloning in Escherichia coli Penicillium chrysogenum

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme-deficient mutant strain: disruption and inactivation of gene lys7 by double-recombination method leads to lysine auxotrophy and accumulation of piperideine-6-carboxylic acid, and with L-lysine as sole N-source and supplementation of DL-alpha-aminoadipic acid, also of pipecolic acid, transformation of the mutant strain with lys7 can restore enzyme activity Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ Penicillium chrysogenum piperidine-6-carboxylic acid and alpha-aminoadipate are precursors for synthesis of alpha-aminoadipate 6-semialdehyde via 3 different pathways, penultimate step in L-lysine biosynthesis saccharopine + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
gene lys7, strain Wis 54-1255
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
wild-type strain Wis 54-1255, cell extract Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ piperidine-6-carboxylic acid and alpha-aminoadipate are precursors for synthesis of alpha-aminoadipate 6-semialdehyde via 3 different pathways, penultimate step in L-lysine biosynthesis Penicillium chrysogenum saccharopine + NADP+ + H2O
-
?
saccharopine + NADP+ + H2O reverse reaction direction used for activity assay, end product is piperidine-6-carboxylic acid in assays using cell extract Penicillium chrysogenum L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
saccharopine reductase
-
Penicillium chrysogenum
SR1
-
Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Penicillium chrysogenum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Penicillium chrysogenum
NADPH
-
Penicillium chrysogenum