Cloned (Comment) | Organism |
---|---|
gene lys, subcloning in Escherichia coli | Penicillium chrysogenum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme-deficient mutant strain: disruption and inactivation of gene lys7 by double-recombination method leads to lysine auxotrophy and accumulation of piperideine-6-carboxylic acid, and with L-lysine as sole N-source and supplementation of DL-alpha-aminoadipic acid, also of pipecolic acid, transformation of the mutant strain with lys7 can restore enzyme activity | Penicillium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ | Penicillium chrysogenum | piperidine-6-carboxylic acid and alpha-aminoadipate are precursors for synthesis of alpha-aminoadipate 6-semialdehyde via 3 different pathways, penultimate step in L-lysine biosynthesis | saccharopine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
gene lys7, strain Wis 54-1255 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22 | - |
wild-type strain Wis 54-1255, cell extract | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ | piperidine-6-carboxylic acid and alpha-aminoadipate are precursors for synthesis of alpha-aminoadipate 6-semialdehyde via 3 different pathways, penultimate step in L-lysine biosynthesis | Penicillium chrysogenum | saccharopine + NADP+ + H2O | - |
? | |
saccharopine + NADP+ + H2O | reverse reaction direction used for activity assay, end product is piperidine-6-carboxylic acid in assays using cell extract | Penicillium chrysogenum | L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
saccharopine reductase | - |
Penicillium chrysogenum |
SR1 | - |
Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Penicillium chrysogenum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Penicillium chrysogenum | |
NADPH | - |
Penicillium chrysogenum |