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Literature summary for 1.5.1.1 extracted from

  • Muramatsu, H.; Mihara, H.; Kakutani, R.; Yasuda, M.; Ueda, M.; Kurihara, T.; Esaki, N.
    The putative malate/lactate dehydrogenase from Pseudomonas putida is an NADPH-dependent DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase involved in the catabolism of D-lysine and D-proline (2005), J. Biol. Chem., 280, 5329-5335.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information enzyme disruption mutant, strain is unable to use D-lysine or D-proline as a sole carbon source Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida involved in the catabolism of D-lysine and D-proline ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.33
-
reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30┬░C Pseudomonas putida
0.67
-
reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADH, pH 8.0, 30┬░C Pseudomonas putida
1.4
-
oxidation of L-pipecolate, cosubstrate NADP+, pH 8.0, 30┬░C Pseudomonas putida
5.4
-
oxidation of L-proline, cosubstrate NADP+, pH 8.0, 30┬░C Pseudomonas putida
51
-
reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30┬░C Pseudomonas putida
92
-
reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADPH, pH 8.0, 30┬░C Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
Pseudomonas putida L-pipecolic acid + NAD(P)+
-
r
DELTA1-pyrroline-2-carboxylate + NAD(P)H at 55% of the rate with DELTA1-piperidine-2-carboxylate Pseudomonas putida L-proline + NAD(P)+
-
r
L-pipecolic acid + NAD(P)+ at 26% of the rate with L-proline Pseudomonas putida DELTA1-piperidine-2-carboxylate + NAD(P)H
-
r
L-proline + NAD(P)+
-
Pseudomonas putida DELTA1-pyrroline-2-carboxylate + NAD(P)H + H+
-
r
additional information involved in the catabolism of D-lysine and D-proline Pseudomonas putida ?
-
?
additional information reverse reactions oxidizing L-proline or L-pipecolic acid are catalyzed at much lower rates. Very poor substrates: trans-4-hydroxy-L-proline, L-thioproline Pseudomonas putida ?
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida
NADH reaction with NADH is less than 1% of that with NADPH Pseudomonas putida
NADP+
-
Pseudomonas putida
NADPH reaction with NADH is less than 1% of that with NADPH Pseudomonas putida