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Literature summary for 1.5.1.1 extracted from

  • Meister, A.; Radhakrishnan, A.N.; Buckley, S.D.
    Enzymatic synthesis of L-pipecolic acid and L-proline (1957), J. Biol. Chem., 229, 789-800.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
NADPH + DELTA1-pyrroline-2-carboxylate Rattus norvegicus
0.043
-
NADH + DELTA1-pyrroline-2-carboxylate Rattus norvegicus
0.06
-
DELTA1-piperidine-2-carboxylate cosubstrate NADH Rattus norvegicus
0.074
-
NADH + DELTA1-piperidine-2-carboxylate Rattus norvegicus
0.082
-
DELTA1-pyrroline-2-carboxylate cosubstrate NADH Rattus norvegicus
0.12
-
DELTA1-pyrroline-2-carboxylate cosubstrate NADPH Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DELTA1-piperidine-2-carboxylate + NAD(P)H Vigna radiata var. radiata
-
L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H Rattus norvegicus
-
L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H Neurospora crassa
-
L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H Pisum sativum
-
L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H Klebsiella aerogenes
-
L-pipecolic acid + NAD(P)+
-
?
DELTA1-pyrroline-2-carboxylate + NAD(P)H Vigna radiata var. radiata
-
L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H Rattus norvegicus
-
L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H Neurospora crassa
-
L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H Pisum sativum
-
L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H Klebsiella aerogenes
-
L-proline + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-
Neurospora crassa
-
-
-
Pisum sativum
-
-
-
Rattus norvegicus
-
-
-
Vigna radiata var. radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
seed germinated seed Vigna radiata var. radiata
-
seed germinated seed Pisum sativum
-
skeletal muscle
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
specific activities in homogenates of various organs Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-2-carboxylate + NADH + H+
-
Rattus norvegicus L-proline + NAD+
-
r
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
Vigna radiata var. radiata L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
Rattus norvegicus L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
Neurospora crassa L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
Pisum sativum L-pipecolic acid + NAD(P)+
-
ir
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
Klebsiella aerogenes L-pipecolic acid + NAD(P)+
-
?
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
Vigna radiata var. radiata L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
Rattus norvegicus L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
Neurospora crassa L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
Pisum sativum L-proline + NAD(P)+
-
ir
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
Klebsiella aerogenes L-proline + NAD(P)+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
DELTA1-piperidine-2-carboxylate Rattus norvegicus
6.8
-
DELTA1-pyrroline-2-carboxylate Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Vigna radiata var. radiata
NADH
-
Rattus norvegicus
NADH
-
Neurospora crassa
NADH
-
Pisum sativum
NADH
-
Klebsiella aerogenes
NADPH
-
Vigna radiata var. radiata
NADPH
-
Rattus norvegicus
NADPH
-
Neurospora crassa
NADPH
-
Pisum sativum
NADPH
-
Klebsiella aerogenes