BRENDA - Enzyme Database
show all sequences of 1.4.99.B3

Structure-function relationships in L-amino acid deaminase, a flavoprotein belonging to a novel class of biotechnologically relevant enzymes

Motta, P.; Molla, G.; Pollegioni, L.; Nardini, M.; J. Biol. Chem. 291, 10457-75 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Cosenzaea myxofaciens
Crystallization (Commentary)
Crystallization (Commentary)
Organism
the overall fold resembles that of known amine or amino acid oxidases. An additional alpha+beta subdomain is placed close to the putative transmembrane alpha-helix and to the active-site entrance, an FAD isoalloxazine ring is exposed to solvent, and a large and accessible active site suits to bind large hydrophobic substrates. The enzyme requires substrate-induced conformational changes of part of the active site, particularly in Arg316 and Phe318, to achieve the correct geometry for catalysis
Cosenzaea myxofaciens
Engineering
Protein Variants
Commentary
Organism
additional information
a deleltion mutant lacking the N-terminal transmembrane alpha-helix, residues 1-27, i almost completely soluble, catalytic activity is below detection
Cosenzaea myxofaciens
General Stability
General Stability
Organism
crude extract, 50% residual activity after 24 h at 25°C
Cosenzaea myxofaciens
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no substrate inhibition up to 100 mM L-phenylalanine
Cosenzaea myxofaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.27
-
L-phenylalanine
pH 7.5, 25°C
Cosenzaea myxofaciens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
presene of a membrane environment is required for activity
Cosenzaea myxofaciens
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44600
-
gel filtration, recombinant N-terminal deletion mutant
Cosenzaea myxofaciens
49900
-
-
Cosenzaea myxofaciens
Organism
Organism
UniProt
Commentary
Textmining
Cosenzaea myxofaciens
A0A158RFS7
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.9
-
pH 7.5, 25°C
Cosenzaea myxofaciens
Storage Stability
Storage Stability
Organism
-20°C, 10% glycerol, 1 month, 50% residual activity
Cosenzaea myxofaciens
-80°C, 10% glycerol, 1 month, 75% residual activity
Cosenzaea myxofaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-cysteine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b
43% of the activitywith L-phenylalanine
-
-
?
L-Dopa + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
3-(3,4-dihydroxyphenyl)-pyruvate + NH3 + 2 reduced cytochrome b
56.7% of the activitywith L-phenylalanine
-
-
?
L-isoleucine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b
43% of the activitywith L-phenylalanine
-
-
?
L-leucine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b
99.2% of the activitywith L-phenylalanine
-
-
?
L-methionine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b
86% of the activitywith L-phenylalanine
-
-
?
L-phenylalanine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
phenylpyruvate + NH3 + 2 reduced cytochrome b
-
-
-
?
L-phenylalanine + H2O + 2,6-dichlorophenolindophenol
-
740746
Cosenzaea myxofaciens
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
membrane-free enzyme can be assayed using 2,6-dichlorophenolindophenol as acceptor
-
-
?
L-phenylalanine ethyl ester + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
phenylpyruvate ethyl ester + NH3 + 2 reduced cytochrome b
33% of the activitywith L-phenylalanine
-
-
?
L-tryptophan + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + 2 reduced cytochrome b
60.5% of the activitywith L-phenylalanine
-
-
?
L-tyrosine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
4-hydroxyphenylpyruvate + NH3 + 2 reduced cytochrome b
13.7% of the activitywith L-phenylalanine
-
-
?
additional information
the enzyme does not use dioxygen to reoxidize reduced FADH2 and does not produce hydrogen peroxide, instead, it uses a cytochrome b-like protein as an electron acceptor. No substrates: D-amino acids
740746
Cosenzaea myxofaciens
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 49900, calculated, recombinant N-terminal deletion mutant
Cosenzaea myxofaciens
Synonyms
Synonyms
Commentary
Organism
L-amino acid deaminase
-
Cosenzaea myxofaciens
LAAD
-
Cosenzaea myxofaciens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Cosenzaea myxofaciens
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
-
negligible activity
Cosenzaea myxofaciens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.5
-
L-phenylalanine
pH 7.5, 25°C
Cosenzaea myxofaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Cosenzaea myxofaciens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
negligible activity bleow and above
Cosenzaea myxofaciens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
an FAD isoalloxazine ring is exposed to solvent
Cosenzaea myxofaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Cosenzaea myxofaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
an FAD isoalloxazine ring is exposed to solvent
Cosenzaea myxofaciens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
the overall fold resembles that of known amine or amino acid oxidases. An additional alpha+beta subdomain is placed close to the putative transmembrane alpha-helix and to the active-site entrance, an FAD isoalloxazine ring is exposed to solvent, and a large and accessible active site suits to bind large hydrophobic substrates. The enzyme requires substrate-induced conformational changes of part of the active site, particularly in Arg316 and Phe318, to achieve the correct geometry for catalysis
Cosenzaea myxofaciens
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
a deleltion mutant lacking the N-terminal transmembrane alpha-helix, residues 1-27, i almost completely soluble, catalytic activity is below detection
Cosenzaea myxofaciens
General Stability (protein specific)
General Stability
Organism
crude extract, 50% residual activity after 24 h at 25°C
Cosenzaea myxofaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no substrate inhibition up to 100 mM L-phenylalanine
Cosenzaea myxofaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.27
-
L-phenylalanine
pH 7.5, 25°C
Cosenzaea myxofaciens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
presene of a membrane environment is required for activity
Cosenzaea myxofaciens
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44600
-
gel filtration, recombinant N-terminal deletion mutant
Cosenzaea myxofaciens
49900
-
-
Cosenzaea myxofaciens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.9
-
pH 7.5, 25°C
Cosenzaea myxofaciens
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10% glycerol, 1 month, 50% residual activity
Cosenzaea myxofaciens
-80°C, 10% glycerol, 1 month, 75% residual activity
Cosenzaea myxofaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-cysteine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b
43% of the activitywith L-phenylalanine
-
-
?
L-Dopa + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
3-(3,4-dihydroxyphenyl)-pyruvate + NH3 + 2 reduced cytochrome b
56.7% of the activitywith L-phenylalanine
-
-
?
L-isoleucine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b
43% of the activitywith L-phenylalanine
-
-
?
L-leucine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b
99.2% of the activitywith L-phenylalanine
-
-
?
L-methionine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b
86% of the activitywith L-phenylalanine
-
-
?
L-phenylalanine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
phenylpyruvate + NH3 + 2 reduced cytochrome b
-
-
-
?
L-phenylalanine + H2O + 2,6-dichlorophenolindophenol
-
740746
Cosenzaea myxofaciens
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
membrane-free enzyme can be assayed using 2,6-dichlorophenolindophenol as acceptor
-
-
?
L-phenylalanine ethyl ester + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
phenylpyruvate ethyl ester + NH3 + 2 reduced cytochrome b
33% of the activitywith L-phenylalanine
-
-
?
L-tryptophan + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + 2 reduced cytochrome b
60.5% of the activitywith L-phenylalanine
-
-
?
L-tyrosine + H2O + 2 cytochrome b
-
740746
Cosenzaea myxofaciens
4-hydroxyphenylpyruvate + NH3 + 2 reduced cytochrome b
13.7% of the activitywith L-phenylalanine
-
-
?
additional information
the enzyme does not use dioxygen to reoxidize reduced FADH2 and does not produce hydrogen peroxide, instead, it uses a cytochrome b-like protein as an electron acceptor. No substrates: D-amino acids
740746
Cosenzaea myxofaciens
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 49900, calculated, recombinant N-terminal deletion mutant
Cosenzaea myxofaciens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Cosenzaea myxofaciens
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
-
negligible activity
Cosenzaea myxofaciens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.5
-
L-phenylalanine
pH 7.5, 25°C
Cosenzaea myxofaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Cosenzaea myxofaciens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
negligible activity bleow and above
Cosenzaea myxofaciens
Other publictions for EC 1.4.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740746
Motta
Structure-function relationshi ...
Cosenzaea myxofaciens
J. Biol. Chem.
291
10457-75
2016
-
-
1
1
1
1
1
1
2
-
2
-
-
4
-
-
-
-
-
-
1
2
11
1
2
1
1
-
1
1
1
-
1
-
-
-
-
-
1
1
1
1
1
-
1
-
1
2
-
2
-
-
-
-
-
-
-
1
2
11
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
740873
Panataleone
-
Purification and characterizat ...
Cosenzaea myxofaciens, Cosenzaea myxofaciens 4482
J. Mol. Catal. B
11
795-803
2001
-
-
1
-
-
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
13
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
13
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391786
Pelmont
L-Amino acid oxidases of Prote ...
Proteus mirabilis
Biochimie
54
1359-1374
1972
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-