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Literature summary for 1.4.99.6 extracted from

  • He, W.; Li, C.; Lu, C.-D.
    Regulation and characterization of the dadRAX locus for D-amino acid catabolism in Pseudomonas aeruginosa PAO1 (2011), J. Bacteriol., 193, 2107-2115 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.99.6

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 D-alanine pH 8.7, 37°C Pseudomonas aeruginosa
0.81
-
 D-histidine pH 8.7, 37°C Pseudomonas aeruginosa
1.07
-
 D-phenylalanine pH 8.7, 37°C Pseudomonas aeruginosa
1.65
-
 D-serine pH 8.7, 37°C Pseudomonas aeruginosa
1.78
-
 D-tyrosine pH 8.7, 37°C Pseudomonas aeruginosa
1.82
-
 D-proline pH 8.7, 37°C Pseudomonas aeruginosa
2.47
-
 D-threonine pH 8.7, 37°C Pseudomonas aeruginosa
3.01
-
 D-valine pH 8.7, 37°C Pseudomonas aeruginosa
5.37
-
 D-arginine pH 8.7, 37°C Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas aeruginosa 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-alanine + H2O + FAD Pseudomonas aeruginosa D-amino acid dehydrogenase activity of broad substrate specificity pyruvate + NH3 + FADH2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HTQ0
-
-

Purification (Commentary)

Purification (Comment) Organism
hexahistidine-tagged DadA Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + FAD D-amino acid dehydrogenase activity of broad substrate specificity Pseudomonas aeruginosa pyruvate + NH3 + FADH2
-
 ?
D-alanine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa pyruvate + NH3 + FADH2
-
 ?
D-arginine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + FADH2
-
 ?
D-histidine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + FADH2
-
 ?
D-phenylalanine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa phenylpyruvate + NH3 + FADH2
-
 ?
D-proline + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa 2-oxopentanoic acid + NH3 + FADH2
-
 ?
D-serine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa 3-hydroxy-2-oxopropanoate + NH3 + FADH2
-
 ?
D-threonine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa 3-hydroxy-2-oxobutanoate + NH3 + FADH2
-
 ?
D-tyrosine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa (4-hydroxyphenyl)pyruvate + NH3 + FADH2
-
 ?
D-valine + H2O + FAD the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity Pseudomonas aeruginosa 2-oxopentanoate + NH3 + FADH2
-
 ?

Synonyms

Synonyms Comment Organism
D-amino acid dehydrogenase DadA
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.68
-
 D-threonine pH 8.7, 37°C Pseudomonas aeruginosa
0.74
-
 D-alanine pH 8.7, 37°C Pseudomonas aeruginosa
0.78
-
 D-valine pH 8.7, 37°C Pseudomonas aeruginosa
0.8
-
 D-serine pH 8.7, 37°C Pseudomonas aeruginosa
0.84
-
 D-histidine pH 8.7, 37°C Pseudomonas aeruginosa
0.97
-
 D-proline pH 8.7, 37°C Pseudomonas aeruginosa
1.1
-
 D-phenylalanine pH 8.7, 37°C Pseudomonas aeruginosa
1.1
-
 D-tyrosine pH 8.7, 37°C Pseudomonas aeruginosa
1.27
-
 D-arginine pH 8.7, 37°C Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 assay at Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa highly up-regulated by L-Ala and D-Ala, followed by L-Val, L-Trp, L-Ser, L-Thr, L-Lys, Gly, L-Cys and several L-amino acids. Expression requires a transcriptional regulator, DadR up

General Information

General Information Comment Organism
malfunction utilization of L-Ala, L-Trp, D-Ala, D-His, D-Phe, D-Ser, D-Thr, and D-Val as sole nitrogen sources is abolished in the dadA mutant Pseudomonas aeruginosa
metabolism D-amino acid dehydrogenase activity of broad substrate specificity Pseudomonas aeruginosa
physiological function involved in catabolism of several D-amino acids Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.236
-
 D-arginine pH 8.7, 37°C Pseudomonas aeruginosa
0.259
-
 D-valine pH 8.7, 37°C Pseudomonas aeruginosa
0.275
-
 D-threonine pH 8.7, 37°C Pseudomonas aeruginosa
0.485
-
 D-serine pH 8.7, 37°C Pseudomonas aeruginosa
0.533
-
 D-proline pH 8.7, 37°C Pseudomonas aeruginosa
0.618
-
 D-tyrosine pH 8.7, 37°C Pseudomonas aeruginosa
1.03
-
 D-phenylalanine pH 8.7, 37°C Pseudomonas aeruginosa
1.04
-
 D-histidine pH 8.7, 37°C Pseudomonas aeruginosa
1.61
-
 D-alanine pH 8.7, 37°C Pseudomonas aeruginosa