KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-arginine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
8.8 | - |
D-histidine | pH 8.7, 25°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HXE3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arginine + phenazine methosulfate | fast substrate D-arginine leads to a dead-end complex of the reduced enzyme and the substrate at high concentrations of D-arginine yielding substrate inhibition, while the overall turnover is partially limited by the release of the iminoarginine product | Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate | overall reaction | ? | |
D-histidine + phenazine methosulfate | slow substrate, the initial Michaelis complex undergoes an isomerization involving multiple conformations that are not all equally catalytically competent for the subsequent oxidation reaction, while the overall turnover is at least partially limited by flavin reduction | Pseudomonas aeruginosa | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
D-histidine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
204 | - |
D-arginine | pH 8.7, 25°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
D-histidine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
3400 | - |
D-arginine | pH 8.7, 25°C | Pseudomonas aeruginosa |