Protein Variants | Comment | Organism |
---|---|---|
E87L | kcat/Km pH-profile of the E87L mutant indicates only a single unprotonated group is required for maximal activity with D-arginine. E87 is the unprotonated group on the enzyme that binds cationic substrates | Pseudomonas aeruginosa |
H48F | residue H48 is not responsible for the observed pKa value | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
D-leucine | wild-type, pH 6.0, 25°C | Pseudomonas aeruginosa | |
4.6 | - |
D-leucine | wild-type, pH 10.0, 25°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HXE3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arginine + phenazine methosulfate | - |
Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate | overall reaction | ? | |
D-leucine + phenazine methosulfate | - |
Pseudomonas aeruginosa | ? + NH3 + reduced phenazine methosulfate | - |
? | |
D-lysine + phenazine methosulfate | - |
Pseudomonas aeruginosa | 6-amino-2-oxohexanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-methionine + phenazine methosulfate | - |
Pseudomonas aeruginosa | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
D-leucine | wild-type, pH 6.0, 25°C | Pseudomonas aeruginosa | |
55 | - |
D-leucine | wild-type, pH 10.0, 25°C | Pseudomonas aeruginosa | |
160 | - |
D-arginine | wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa | |
190 | - |
D-methionine | wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa | |
550 | - |
D-Lysine | wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
D-leucine | wild-type, pH 6.0, 25°C | Pseudomonas aeruginosa | |
12 | - |
D-leucine | wild-type, pH 10.0, 25°C | Pseudomonas aeruginosa | |
36 | - |
D-methionine | wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa | |
140 | - |
D-arginine | wild-type, pH-independent limiting value at high pH, pH not specified in the publication, 6°C | Pseudomonas aeruginosa | |
150 | - |
D-arginine | mutant E87L, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa | |
580 | - |
D-Lysine | wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa | |
1100 | - |
D-arginine | mutant H48F, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa | |
2600 | - |
D-arginine | wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa |