BRENDA - Enzyme Database
show all sequences of 1.4.9.2

Aromatic amine dehydrogenase, a second tryptophan tryptophylquinone enzyme

Govindaraj, S.; Eisenstein, E.; Jones, L.H.; Sanders-Loehr, J.; Chistoserdov, A.Y.; Davidson, V.L.; Edwards, S.L.; J. Bacteriol. 176, 2922-2929 (1994)

Data extracted from this reference:

General Stability
General Stability
Organism
stable against guanidine hydrochloride up to 2 M
Alcaligenes faecalis
Inhibitors
Inhibitors
Commentary
Organism
Structure
aminoguanidine
-
Alcaligenes faecalis
hydrazine
-
Alcaligenes faecalis
hydroxylamine
-
Alcaligenes faecalis
Iproniazid
-
Alcaligenes faecalis
isoniazid
-
Alcaligenes faecalis
phenylhydrazine
-
Alcaligenes faecalis
Semicarbazide
-
Alcaligenes faecalis
tyramine
substrate inhibition
Alcaligenes faecalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0054
-
tyramine
-
Alcaligenes faecalis
0.25
-
phenazine methosulfate
-
Alcaligenes faecalis
0.45
-
phenazine ethosulfate
-
Alcaligenes faecalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
alpha2beta2, 2 * 39000 + 2 * 18000, SDS-PAGE
Alcaligenes faecalis
39000
-
alpha2beta2, 2 * 39000 + 2 * 18000, SDS-PAGE
Alcaligenes faecalis
108900
-
sedimentation equilibrium
Alcaligenes faecalis
114000
-
estimated from subunit molecular weights
Alcaligenes faecalis
Organism
Organism
UniProt
Commentary
Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis IFO 14479
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Alcaligenes faecalis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.39
-
cell extract from cells grown in beta-phenylethylamine
Alcaligenes faecalis
14.3
-
after purification
Alcaligenes faecalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
phenazine ethosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
phenazine ethosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis IFO 14479
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
phenazine methosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
phenazine methosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis IFO 14479
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
tyramine + acceptor + H2O
electron acceptor: phenazine methosulfate or phenazine ethosulfate
396592
Alcaligenes faecalis
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
tyramine + acceptor + H2O
electron acceptor: phenazine methosulfate or phenazine ethosulfate
396592
Alcaligenes faecalis IFO 14479
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
alpha2beta2, 2 * 39000 + 2 * 18000, SDS-PAGE
Alcaligenes faecalis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
60
full activity after incubation for 20 min
Alcaligenes faecalis
Cofactor
Cofactor
Commentary
Organism
Structure
tryptophan tryptophylquinone
-
Alcaligenes faecalis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0084
-
isoniazid
-
Alcaligenes faecalis
0.186
-
Iproniazid
-
Alcaligenes faecalis
1.08
-
tyramine
substrate inhibition
Alcaligenes faecalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
tryptophan tryptophylquinone
-
Alcaligenes faecalis
General Stability (protein specific)
General Stability
Organism
stable against guanidine hydrochloride up to 2 M
Alcaligenes faecalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
aminoguanidine
-
Alcaligenes faecalis
hydrazine
-
Alcaligenes faecalis
hydroxylamine
-
Alcaligenes faecalis
Iproniazid
-
Alcaligenes faecalis
isoniazid
-
Alcaligenes faecalis
phenylhydrazine
-
Alcaligenes faecalis
Semicarbazide
-
Alcaligenes faecalis
tyramine
substrate inhibition
Alcaligenes faecalis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0084
-
isoniazid
-
Alcaligenes faecalis
0.186
-
Iproniazid
-
Alcaligenes faecalis
1.08
-
tyramine
substrate inhibition
Alcaligenes faecalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0054
-
tyramine
-
Alcaligenes faecalis
0.25
-
phenazine methosulfate
-
Alcaligenes faecalis
0.45
-
phenazine ethosulfate
-
Alcaligenes faecalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
alpha2beta2, 2 * 39000 + 2 * 18000, SDS-PAGE
Alcaligenes faecalis
39000
-
alpha2beta2, 2 * 39000 + 2 * 18000, SDS-PAGE
Alcaligenes faecalis
108900
-
sedimentation equilibrium
Alcaligenes faecalis
114000
-
estimated from subunit molecular weights
Alcaligenes faecalis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Alcaligenes faecalis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.39
-
cell extract from cells grown in beta-phenylethylamine
Alcaligenes faecalis
14.3
-
after purification
Alcaligenes faecalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
phenazine ethosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
phenazine ethosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis IFO 14479
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
phenazine methosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
phenazine methosulfate + H2O + 2,6-dichlorophenol indophenol
-
396592
Alcaligenes faecalis IFO 14479
? + NH3 + reduced 2,6-dichlorophenol indophenol
-
-
-
-
tyramine + acceptor + H2O
electron acceptor: phenazine methosulfate or phenazine ethosulfate
396592
Alcaligenes faecalis
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
tyramine + acceptor + H2O
electron acceptor: phenazine methosulfate or phenazine ethosulfate
396592
Alcaligenes faecalis IFO 14479
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
alpha2beta2, 2 * 39000 + 2 * 18000, SDS-PAGE
Alcaligenes faecalis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
60
full activity after incubation for 20 min
Alcaligenes faecalis
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
685206
Hothi
Catalysis by the isolated tryp ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochemistry
47
183-194
2008
-
-
-
-
-
-
1
-
-
-
-
-
-
10
-
-
1
-
-
1
-
-
8
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685188
Hothi
Isotope effects reveal that pa ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochemistry
46
9250-9259
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
1
-
-
4
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687589
Roujeinikova
New insights into the reductiv ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
J. Biol. Chem.
282
23766-23777
2007
-
-
-
-
-
-
-
-
-
-
2
-
-
9
-
-
1
-
-
-
-
-
8
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672092
Sukumar
Crystal structure of an electr ...
Alcaligenes faecalis
Biochemistry
45
13500-13510
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673559
Hothi
Tryptophan tryptophylquinone c ...
Alcaligenes faecalis
FEBS J.
273
430
2006
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
1
-
-
1
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
674733
Roujeinikova
Atomic level insight into the ...
Alcaligenes faecalis
J. Biol. Chem.
281
40264-40272
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677123
Massgrau
Atomic description of an enzym ...
Alcaligenes faecalis
Science
312
237-241
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655062
Kondo
Purification and characterizat ...
Achromobacter xylosoxidans, Achromobacter xylosoxidans IFO13495
Biosci. Biotechnol. Biochem.
68
1921-1928
2004
-
-
-
-
-
-
15
4
-
-
3
2
-
3
-
-
1
1
-
1
1
1
23
1
2
1
1
3
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
15
-
4
-
-
3
2
-
-
-
1
-
1
1
1
23
1
1
1
3
-
1
-
1
-
-
-
-
-
-
-
396596
Chistoserdov
Cloning, sequencing and mutage ...
Achromobacter xylosoxidans, Alcaligenes faecalis
Microbiology
147
2195-2202
2001
-
-
-
-
-
-
-
-
1
-
2
-
-
5
-
-
-
-
-
-
2
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396595
Hyun
Gated and ungated electron tra ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
J. Biol. Chem.
274
29081-29086
1999
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715340
Zhu
Localization of periplasmic re ...
Alcaligenes faecalis
J. Bacteriol.
181
6540-6542
1999
-
-
-
1
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714515
Adachi
-
Characterization of quinohemop ...
Pseudomonas putida, Pseudomonas putida IFO 15366
Biosci. Biotechnol. Biochem.
62
469-478
1998
-
-
-
1
-
-
4
-
-
-
4
-
-
2
-
-
-
-
-
-
1
-
6
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396593
Hyun
Electron transfer reactions be ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochemistry
34
12249-12254
1995
-
-
-
-
-
-
-
2
-
-
-
2
-
10
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
396594
Hyun
Unusually large isotope effect ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochim. Biophys. Acta
1251
198-200
1995
-
-
-
-
-
-
-
2
-
-
-
-
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
715426
Edwards
Spectroscopic evidence for a c ...
Alcaligenes faecalis
J. Biol. Chem.
270
4293-4298
1995
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396592
Govindaraj
Aromatic amine dehydrogenase, ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
J. Bacteriol.
176
2922-2929
1994
-
-
-
-
-
1
8
3
-
-
4
-
-
9
-
-
1
-
-
-
2
-
6
1
-
-
-
1
-
-
-
-
1
3
-
-
-
-
-
1
-
-
1
-
8
3
3
-
-
4
-
-
-
-
1
-
-
2
-
6
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288289
Cuskey
Initial catabolism of aromatic ...
Pseudomonas aeruginosa
J. Bacteriol.
169
2398-2404
1987
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396589
Nozaki
Aromatic amine dehydrogenase f ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Methods Enzymol.
142
650-655
1987
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396591
Iwaki
Crystallization and properties ...
Pseudomonas sp.
Arch. Biochem. Biophys.
220
253-262
1983
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