Protein Variants | Comment | Organism |
---|---|---|
alphaF55A | 1656fold increase in Km-value for methylamine compared to wild-type enzyme, 484fold increase in Km-value for ethylamine compared to wild-type enzyme, 36fold increase in Km-value for propylamine compared to wild-type enzyme, 3.6fold decrease in Km-value for butylamine compared to wild-type enzyme, 53.2fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 34.3fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 54.3fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme | Paracoccus denitrificans |
alphaF55I | 1.2fold decrease in Km-value for methylamine compared to wild-type enzyme, 18.9fold increase in Km-value for ethylamine compared to wild-type enzyme, 5.6fold increase in Km-value for propylamine compared to wild-type enzyme, 4.2fold decrease in Km-value for butylamine compared to wild-type enzyme, 10fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 7.3fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 6.7fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzym. Ability to discriminate between amines of different chain length is abolished | Paracoccus denitrificans |
betaI107N | 27.8fold increase in Km-value for methylamine compared to wild-type enzyme, 44.2fold increase in Km-value for ethylamine compared to wild-type enzyme, 8.5fold decrease in Km-value for propylamine compared to wild-type enzyme, 124fold decrease in Km-value for butylamine compared to wild-type enzyme, 62.5fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 23.2fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 5.6fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme. Mutant enzyme exhibity a strong preference for 1-aminopentane compared to strong preference for methylamine of the wild-type enzyme | Paracoccus denitrificans |
betaI107V | mutant enzyme exhibits a strong preference for propylamine compared to strong preference for methylamine of the wild-type enzyme. 7.7fold increase in Km-value for methylamine compared to wild-type enzyme, 17.9fold increase in Km-value for ethylamine compared to wild-type enzyme, 6fold decrease in Km-value for propylamine compared to wild-type enzyme, 9.9fold decrease in Km-value for butylamine compared to wild-type enzyme, 19.2fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 4.2fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 1.3fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
0.006 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
0.007 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
0.007 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
0.008 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
0.009 | - |
methylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
0.019 | - |
ethylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
0.021 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
0.031 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
0.036 | - |
Propylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
0.047 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
0.057 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
0.06 | - |
methylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
0.068 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
0.069 | - |
methylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
0.088 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
0.099 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
0.13 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
0.17 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
0.2 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
0.24 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
0.25 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
0.25 | - |
methylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
0.29 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
0.34 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
0.36 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
0.37 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
0.38 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
0.72 | - |
1,6-diaminohexane | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
0.84 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
0.87 | - |
Butylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
1.3 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
2.5 | - |
1-aminopentane | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
9.2 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
14.9 | - |
methylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,6-diaminohexane + acceptor + H2O | acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol | Paracoccus denitrificans | ? | - |
? | |
1,7-diaminoheptane + acceptor + H2O | acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol | Paracoccus denitrificans | ? | - |
? | |
1-aminopentane + acceptor + H2O | acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol | Paracoccus denitrificans | pentanal + NH3 + reduced acceptor | - |
? | |
butylamine + acceptor + H2O | acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol | Paracoccus denitrificans | butanal + NH3 + reduced acceptor | - |
? | |
ethylamine + acceptor + H2O | acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol | Paracoccus denitrificans | acetaldehyde + NH3 + reduced acceptor | - |
? | |
methylamine + acceptor + H2O | acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol | Paracoccus denitrificans | formaldehyde + NH3 + reduced acceptor | - |
? | |
propylamine + acceptor + H2O | acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol | Paracoccus denitrificans | propionaldehyde + NH3 + reduced acceptor | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
methylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
3.1 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
3.4 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
3.4 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
3.4 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
4.1 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
4.2 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
4.2 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
4.5 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
5.2 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
5.4 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
5.4 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
6.2 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
14 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
15 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme alphaF55I | Paracoccus denitrificans | |
15 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
16 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
17 | - |
1,6-diaminohexane | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
17 | - |
1-aminopentane | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
19 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
20 | - |
1-aminopentane | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
20 | - |
methylamine | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
22 | - |
Butylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
23 | - |
ethylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
24 | - |
Propylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
24 | - |
ethylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
26 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme betaI107V | Paracoccus denitrificans | |
27 | - |
Propylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
27 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
30 | - |
methylamine | pH 7.5, 30°C, wild-type enzyme | Paracoccus denitrificans | |
32 | - |
1,7-Diaminoheptane | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
34 | - |
Butylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
34 | - |
methylamine | pH 7.5, 30°C, mutant enzyme betaI107N | Paracoccus denitrificans | |
43 | - |
1,6-diaminohexane | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans | |
77 | - |
methylamine | pH 7.5, 30°C, mutant enzyme alphaF55A | Paracoccus denitrificans |