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Literature summary for 1.4.5.1 extracted from
- Solubilization, purification and characterization of D-alanine dehydrogenase from Pseudomonas aeruginosa and effects of solubilization on its properties (1987), Biochimie, 69, 63-69.
General Stability
| General Stability | Organism |
|---|---|
| association with the membrane bilayer protects the enzyme against thermal denaturation | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
D-alanine | purified preparation | Pseudomonas aeruginosa |  |
| 26 | - |
D-alanine | solubilized enzyme | Pseudomonas aeruginosa | |
| 53 | - |
D-alanine | native enzyme | Pseudomonas aeruginosa | |
| 71 | - |
D-glutamine | - |
Pseudomonas aeruginosa | |
| 200 | - |
D-cycloserine | - |
Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Pseudomonas aeruginosa | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.653 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O | 81% of the activity with D-alanine | Pseudomonas aeruginosa | 2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-alanine + oxidized 2,6-dichloroindophenol + H2O | - |
Pseudomonas aeruginosa | pyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-arginine + oxidized 2,6-dichlorophenolindophenol + H2O | 41% of the activity with D-alanine | Pseudomonas aeruginosa | 2-oxo-5-guanidinopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-asparagine + oxidized 2,6-dichlorophenolindophenol + H2O | 96% of the activity with D-alanine | Pseudomonas aeruginosa | 2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-cycloserine + oxidized 2,6-dichloroindophenol + H2O | as a active as D-alanine | Pseudomonas aeruginosa | ? | - |
? | |
| D-glutamine + oxidized 2,6-dichloroindophenol + H2O | 52% of the activity with D-alanine | Pseudomonas aeruginosa | 4-carbamoyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-methionine + oxidized 2,6-dichlorophenolindophenol + H2O | 70% of the activity with D-alanine | Pseudomonas aeruginosa | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O | 63% of the activity with D-alanine | Pseudomonas aeruginosa | phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-proline + oxidized 2,6-dichlorophenolindophenol | 19% of the activity with D-alanine | Pseudomonas aeruginosa | DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-valine + oxidized 2,6-dichlorophenolindophenol + H2O | 26% of the activity with D-alanine | Pseudomonas aeruginosa | 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 49000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
association with the membrane bilayer protects the enzyme against thermal denaturation | Pseudomonas aeruginosa |
| 37 | - |
60 min, solubilized enzyme loses 98% of its initial activity, the native enzyme loses 47% of its activity | Pseudomonas aeruginosa |
| 42 | - |
10 min, solubilized enzyme loses 95% of its activity, the native enzyme loses 49% of its activity | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Pseudomonas aeruginosa | |
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