Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.5.1 extracted from

  • Tanigawa, M.; Shinohara, T.; Saito, M.; Nishimura, K.; Hasegawa, Y.; Wakabayashi, S.; Ishizuka, M.; Nagata, Y.D
    D-Amino acid dehydrogenase from Helicobacter pylori NCTC 11637 (2010), Amino Acids, 38, 247-255.
    View publication on PubMed
Search for more literature for 1.4.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
benzoate 0.1 mM, 98% inhibition Helicobacter pylori
iodoacetamide 0.1 mM, 66% inhibition Helicobacter pylori
iodoacetic acid 0.1 mM, 62% inhibition Helicobacter pylori
additional information EDTA, Mg2+ and Ca2+ have no effect on DadA activity Helicobacter pylori
p-hydroxybenzoic acid 0.1 mM, 79% inhibition Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
 oxidized coenzyme Q1 pH 8.0, 37°C Helicobacter pylori
40.2
-
 D-proline pH 8.0, 37°C Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ and Ca2+ have no effect on DadA activity Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Helicobacter pylori
46040
-
 x * 46040, calculated from sequence Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori A3KEZ1 NCTC 11637
-

Purification (Commentary)

Purification (Comment) Organism
from Helicobacter pylori and recombinant Escherichia coli cells Helicobacter pylori

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.22
-
-
 Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala + oxidized coenzyme Q1 + H2O specific activity is 37% of that with D-proline Helicobacter pylori pyruvate + NH3 + reduced coenzyme Q1
-
 ?
D-Phe + oxidized coenzyme Q1 + H2O specific activity is 20% of that with D-proline Helicobacter pylori phenylpyruvate + NH3 + reduced coenzyme Q1
-
 ?
D-proline + oxidized coenzyme Q1 + H2O
-
 Helicobacter pylori DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
-
 ?
D-Ser + oxidized coenzyme Q1 + H2O specific activity is 7% of that with D-proline Helicobacter pylori 3-hydroxy-2-oxopropanoate + NH3 + reduced coenzyme Q1
-
 ?
additional information activity with D-Val, D-Met, D-Glu, D-Asp, L-Pro and L-Ala is below 5% of the activity with D-Pro Helicobacter pylori ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Helicobacter pylori
? x * 46040, calculated from sequence Helicobacter pylori

Synonyms

Synonyms Comment Organism
DadA
-
 Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Helicobacter pylori

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 25°C: about 50% of maximal activity, 55°C: about 65% of maximal activity Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Helicobacter pylori

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 pH 7.5: about 50% of maximal activity, pH 8.5: about 45% of maximal activity Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
FAD flavoenzyme Helicobacter pylori