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Literature summary for 1.4.5.1 extracted from

  • Jones, H.; Venables, W.A.
    Effects of solubilization on some properties of the membrane-bound respiratory enzyme D-amino acid dehydrogenase of Escherichia coli (1983), FEBS Lett., 151, 189-192.
    View publication on PubMed
Search for more literature for 1.4.5.1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O 38% of the activity compared to D-alanine Escherichia coli 2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-alanine + oxidized 2,6-dichloroindophenol + H2O
-
 Escherichia coli pyruvate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-asparagine + oxidized 2,6-dichlorophenolindophenol + H2O 26% of the activity compared to D-alanine Escherichia coli 2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
D-histidine + oxidized 2,6-dichloroindophenol + H2O 8.5% of the activity compared to D-alanine Escherichia coli 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-methionine + oxidized 2,6-dichloroindophenol + H2O 35% of the activity compared to D-alanine Escherichia coli 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
D-serine + oxidized 2,6-dichlorophenolindophenol + H2O 20% of the activity compared to D-alanine Escherichia coli 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 integration of the enzyme within the membrane bilayer confers direct protection against thermal denaturation at 37°C Escherichia coli
37
-
 10 min, the solubilized enzyme loses 90% of initial activity, the native enzyme loses 20% of initial activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 substrate: D-alanine, solubilized enzyme Escherichia coli
8.9
-
 substrate: D-alanine, native enzyme Escherichia coli