BRENDA - Enzyme Database
show all sequences of 1.4.3.8

Metabolism of ethanolamine, an ethanoloxidase

Narrod, S.A.; Jakoby, W.B.; J. Biol. Chem. 239, 2189-2193 (1964)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no stimulation of activity by FAD, pyridoxalphosphate, NAD+, NADP+, FMN or tetrazolium dyes
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Pseudomonas sp.
cysteine
-
Pseudomonas sp.
glutathione
-
Pseudomonas sp.
hydrazine
-
Pseudomonas sp.
hydroxylamine
-
Pseudomonas sp.
Iproniazide
-
Pseudomonas sp.
isoniazide
low inhibition
Pseudomonas sp.
Quinacrine
-
Pseudomonas sp.
Semicarbazide
-
Pseudomonas sp.
Sodium bisulfite
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
-
ethanolamine
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ethanolamine + H2O + O2
Pseudomonas sp.
aerobic catabolism of ethanolamine
glycolaldehyde + NH3 + H2O2
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
Bacterium ATCC 13796, Arthrobacter sp.
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
enzyme more active in larvae than in adults
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0621
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-amino-2-propanol + H2O + O2
18% of the activity with ethanolamine
391966
Pseudomonas sp.
2-hydroxy-propionic aldehyde + NH3 + H2O2
-
-
-
?
3-amino-1-propanol + H2O + O2
56% of the activity with ethanolamine
391966
Pseudomonas sp.
3-hydroxy-propionic aldehyde + NH3 + H2O2
-
-
-
?
ethanolamine + H2O + O2
under aerobic conditions
391966
Pseudomonas sp.
glycolaldehyde + NH3 + H2O2
-
-
-
ir
ethanolamine + H2O + O2
aerobic catabolism of ethanolamine
391966
Pseudomonas sp.
glycolaldehyde + NH3 + H2O2
-
-
-
ir
additional information
no activity with: ethylamine, n-propylamine, putrescine, choline and phosphoethanolamine
391966
Pseudomonas sp.
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.8
half-maximal activities at pH 7 and pH 9.8
Pseudomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no stimulation of activity by FAD, pyridoxalphosphate, NAD+, NADP+, FMN or tetrazolium dyes
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Pseudomonas sp.
cysteine
-
Pseudomonas sp.
glutathione
-
Pseudomonas sp.
hydrazine
-
Pseudomonas sp.
hydroxylamine
-
Pseudomonas sp.
Iproniazide
-
Pseudomonas sp.
isoniazide
low inhibition
Pseudomonas sp.
Quinacrine
-
Pseudomonas sp.
Semicarbazide
-
Pseudomonas sp.
Sodium bisulfite
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
-
ethanolamine
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ethanolamine + H2O + O2
Pseudomonas sp.
aerobic catabolism of ethanolamine
glycolaldehyde + NH3 + H2O2
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
enzyme more active in larvae than in adults
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0621
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-amino-2-propanol + H2O + O2
18% of the activity with ethanolamine
391966
Pseudomonas sp.
2-hydroxy-propionic aldehyde + NH3 + H2O2
-
-
-
?
3-amino-1-propanol + H2O + O2
56% of the activity with ethanolamine
391966
Pseudomonas sp.
3-hydroxy-propionic aldehyde + NH3 + H2O2
-
-
-
?
ethanolamine + H2O + O2
under aerobic conditions
391966
Pseudomonas sp.
glycolaldehyde + NH3 + H2O2
-
-
-
ir
ethanolamine + H2O + O2
aerobic catabolism of ethanolamine
391966
Pseudomonas sp.
glycolaldehyde + NH3 + H2O2
-
-
-
ir
additional information
no activity with: ethylamine, n-propylamine, putrescine, choline and phosphoethanolamine
391966
Pseudomonas sp.
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.8
half-maximal activities at pH 7 and pH 9.8
Pseudomonas sp.
Other publictions for EC 1.4.3.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742141
Pereira
Engineering a novel biosynthe ...
Arthrobacter sp. FERM P-06240
Biotechnol. Bioeng.
113
376-383
2016
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391967
Kulkarni
Ethanolamine oxidase from the ...
Phormia regina
Comp. Biochem. Physiol. B
44
407-422
1973
1
-
-
-
-
1
14
1
2
3
-
-
-
3
-
-
1
-
-
3
1
1
1
-
-
1
1
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
1
-
14
-
1
2
3
-
-
-
-
-
1
-
3
1
1
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
391965
Narrod
-
Ethanolamine oxidase ...
Pseudomonas sp.
Methods Enzymol.
8
354-357
1966
-
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
1
-
-
-
1
1
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
-
-
-
-
-
-
-
1
-
-
1
1
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
391966
Narrod
Metabolism of ethanolamine, an ...
Pseudomonas sp.
J. Biol. Chem.
239
2189-2193
1964
1
-
-
-
-
-
10
1
-
-
-
1
-
1
-
-
1
-
-
1
1
-
5
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
1
-
1
1
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-