BRENDA - Enzyme Database
show all sequences of 1.4.3.7

Purification and properties of D-glutamate oxidase from Candida boidinii 2201

Fukunaga, S.; Yno, S.; Takahashi, M.; Taguchi, S.; Kera, Y.; Odani, S.; Yamada, R.H.; J. Ferment. Bioeng. 85, 579-583 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-malate
competitive inhibitor
[Candida] boidinii
Glutarate
competitive inhibitor
[Candida] boidinii
m-tartrate
competitive inhibitor
[Candida] boidinii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
[Candida] boidinii
45000
-
1 * 45000, SDS-PAGE
[Candida] boidinii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-aspartate + H2O + O2
[Candida] boidinii
poorer substrate than D-glutamate
oxaloacetate + NH3 + H2O2
-
[Candida] boidinii
?
D-glutamate + H2O + O2
[Candida] boidinii
better substrate than D-aspartate
2-oxoglutarate + NH3 + H2O2
-
[Candida] boidinii
?
Organism
Organism
UniProt
Commentary
Textmining
[Candida] boidinii
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
to homogeneity
[Candida] boidinii
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
[Candida] boidinii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-aspartate + H2O + O2
poorer substrate than D-glutamate
395286
[Candida] boidinii
oxaloacetate + NH3 + H2O2
-
395286
[Candida] boidinii
?
D-glutamate + H2O + O2
better substrate than D-aspartate
395286
[Candida] boidinii
2-oxoglutarate + NH3 + H2O2
-
395286
[Candida] boidinii
?
N-methyl-D-aspartate + H2O + O2
much poorer substrate than D-glutamate or D-aspartate
395286
[Candida] boidinii
oxaloacetate + methylamine + H2O2
-
395286
[Candida] boidinii
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
[Candida] boidinii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
[Candida] boidinii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
[Candida] boidinii
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
cannot be replaced by FMN
[Candida] boidinii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
cannot be replaced by FMN
[Candida] boidinii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-malate
competitive inhibitor
[Candida] boidinii
Glutarate
competitive inhibitor
[Candida] boidinii
m-tartrate
competitive inhibitor
[Candida] boidinii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
[Candida] boidinii
45000
-
1 * 45000, SDS-PAGE
[Candida] boidinii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-aspartate + H2O + O2
[Candida] boidinii
poorer substrate than D-glutamate
oxaloacetate + NH3 + H2O2
-
[Candida] boidinii
?
D-glutamate + H2O + O2
[Candida] boidinii
better substrate than D-aspartate
2-oxoglutarate + NH3 + H2O2
-
[Candida] boidinii
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity
[Candida] boidinii
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
[Candida] boidinii
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-aspartate + H2O + O2
poorer substrate than D-glutamate
395286
[Candida] boidinii
oxaloacetate + NH3 + H2O2
-
395286
[Candida] boidinii
?
D-glutamate + H2O + O2
better substrate than D-aspartate
395286
[Candida] boidinii
2-oxoglutarate + NH3 + H2O2
-
395286
[Candida] boidinii
?
N-methyl-D-aspartate + H2O + O2
much poorer substrate than D-glutamate or D-aspartate
395286
[Candida] boidinii
oxaloacetate + methylamine + H2O2
-
395286
[Candida] boidinii
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
[Candida] boidinii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
[Candida] boidinii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
[Candida] boidinii
Other publictions for EC 1.4.3.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
395286
Fukunaga
Purification and properties of ...
[Candida] boidinii
J. Ferment. Bioeng.
85
579-583
1998
-
-
-
-
-
-
3
-
-
-
2
2
-
3
-
-
1
-
-
2
-
-
3
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
2
2
-
-
-
1
-
2
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395287
Urich
-
D-Glutamtoxydase aus der Anten ...
Faxonius limosus
Z. Naturforsch. B
23
1508-1511
1968
-
-
-
-
-
1
6
2
-
-
-
2
-
1
-
-
1
-
-
1
1
1
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
2
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
395288
Rocca
Purification and properties of ...
Octopus vulgaris
Arch. Biochem. Biophys.
77
336-349
1958
1
-
-
-
-
-
5
2
-
-
-
4
-
3
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
5
-
2
-
-
-
4
-
-
-
1
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-