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Literature summary for 1.4.3.5 extracted from

  • Barile, A.; Tramonti, A.; di Salvo, M.L.; Nogues, I.; Nardella, C.; Malatesta, F.; Contestabile, R.
    Allosteric feedback inhibition of pyridoxine 5-phosphate oxidase from Escherichia coli (2019), J. Biol. Chem., 294, 15593-15603 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli MDS00 strain cells lacking pdxH gene Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
pyridoxal 5'-phosphate product inhibition is of a mixed-type nature and results from binding of this vitamer at an allosteric site Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxine 5'-phosphate + O2 Escherichia coli the enzyme catalyzes the last step is pyridoxine 5'-phosphate oxidation to pyridoxal 5'-phosphate. The enzyme plays a pivotal role in controlling intracellular homeostasis and bioavailability of pyridoxal 5'-phosphate pyridoxal 5'-phosphate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxine 5'-phosphate + O2
-
 Escherichia coli pyridoxal 5'-phosphate + H2O2
-
 ?
pyridoxine 5'-phosphate + O2 the enzyme catalyzes the last step is pyridoxine 5'-phosphate oxidation to pyridoxal 5'-phosphate. The enzyme plays a pivotal role in controlling intracellular homeostasis and bioavailability of pyridoxal 5'-phosphate Escherichia coli pyridoxal 5'-phosphate + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
PdxH
-
 Escherichia coli
PNPO
-
 Escherichia coli
pyridoxine 5'-phosphate oxidase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 pyridoxine 5'-phosphate 37°C, pH 7.6 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN FMN-dependent enzyme Escherichia coli

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the last step is pyridoxine 5'-phosphate oxidation to pyridoxal 5'-phosphate. The enzyme plays a pivotal role in controlling intracellular homeostasis and bioavailability of pyridoxal 5'-phosphate Escherichia coli