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Literature summary for 1.4.3.5 extracted from

  • Mashalidis, E.H.; Mukherjee, T.; Sled?, P.; Matak-Vinkovi?, D.; Boshoff, H.; Abell, C.; Barry, C.E.
    Rv2607 from Mycobacterium tuberculosis is a pyridoxine 5-phosphate oxidase with unusual substrate specificity (2011), PLoS ONE, 6, e27643.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 pyridoxine 5'-phosphate in 25 mM potassium phosphate, pH 7.8 and 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 27000, SDS-PAGE Mycobacterium tuberculosis
54000
-
 gel filtration Mycobacterium tuberculosis
55380
-
 nano-ESI mass spectroscopy Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis the enzyme does not recognize pyridoxamine 5'-phosphate as substrate ?
-
 ?
additional information Mycobacterium tuberculosis H37Rv the enzyme does not recognize pyridoxamine 5'-phosphate as substrate ?
-
 ?
pyridoxine 5'-phosphate + H2O + O2 Mycobacterium tuberculosis
-
 pyridoxal 5'-phosphate + H2O2
-
 ?
pyridoxine 5'-phosphate + H2O + O2 Mycobacterium tuberculosis H37Rv
-
 pyridoxal 5'-phosphate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not recognize pyridoxamine 5'-phosphate as substrate Mycobacterium tuberculosis ?
-
 ?
additional information the enzyme does not recognize pyridoxamine 5'-phosphate as substrate Mycobacterium tuberculosis H37Rv ?
-
 ?
pyridoxine 5'-phosphate + H2O + O2
-
 Mycobacterium tuberculosis pyridoxal 5'-phosphate + H2O2
-
 ?
pyridoxine 5'-phosphate + H2O + O2
-
 Mycobacterium tuberculosis H37Rv pyridoxal 5'-phosphate + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 27000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
PNPOx
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 pyridoxine 5'-phosphate in 25 mM potassium phosphate, pH 7.8 and 25°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
FMN contains one FMN molecule per dimer Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.028
-
 pyridoxine 5'-phosphate in 25 mM potassium phosphate, pH 7.8 and 25°C Mycobacterium tuberculosis