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Literature summary for 1.4.3.5 extracted from

  • Kazarinoff, M.N.; McCormick, D.B.
    N-(5-phospho-4-pyridoxyl)amines as substrates for pyridoxine (pyridoxamine) 5-phosphate oxidase (1973), Biochem. Biophys. Res. Commun., 52, 440-446.
    View publication on PubMed
Search for more literature for 1.4.3.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 pyridoxamine 5'-phosphate pH 8.0 Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus
native enzyme 556fold from liver by acid and ethanol precipitations, anion exchange chromatography, gel filtration, and calcium phosphate gel chromatography Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2 reaction mechanism Oryctolagus cuniculus
a
pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2 reaction mechanism Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.156
-
-
 Oryctolagus cuniculus
0.157
-
 purified enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-phospho-pyridoxyl-2-aminobutyrate + H2O + O2 110% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + 2-aminobutyrate + H2O2
-
 ?
5'-phospho-pyridoxyl-3-alanine + H2O + O2 85% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + alanine + H2O2
-
 ?
5'-phospho-pyridoxyl-benzylamine + H2O + O2 120% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + benzylamine + H2O2
-
 ?
5'-phospho-pyridoxyl-D-2-aminobutyrate + H2O + O2 120% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-2-aminobutyrate + H2O2
-
 ?
5'-phospho-pyridoxyl-D-alanine + H2O + O2 130% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-alanine + H2O2
-
 ?
5'-phospho-pyridoxyl-D-leucine + H2O + O2 120% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-leucine + H2O2
-
 ?
5'-phospho-pyridoxyl-D-tyrosine + H2O + O2 55% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-tyrosine + H2O2
-
 ?
5'-phospho-pyridoxyl-glycine + H2O + O2 110% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + glycine + H2O2
-
 ?
5'-phospho-pyridoxyl-L-2-aminobutyrate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-2-aminobutyrate + H2O2
-
 ?
5'-phospho-pyridoxyl-L-alanine + H2O + O2 140% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-alanine + H2O2
-
 ?
5'-phospho-pyridoxyl-L-leucine + H2O + O2 86% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-leucine + H2O2
-
 ?
5'-phospho-pyridoxyl-L-phenylalanine + H2O + O2 54% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-phenylalanine + H2O2
-
 ?
5'-phospho-pyridoxyl-L-serine + H2O + O2 39% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-serine + H2O2
-
 ?
5'-phospho-pyridoxyl-L-tryptophan + H2O + O2 18% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-tryptophan + H2O2
-
 ?
5'-phospho-pyridoxyl-L-tyrosine + H2O + O2 57% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-tyrosine + H2O2
-
 ?
additional information a 5'-phosphate moiety is absolutely required for activity, substrate specificities with various N-(phosphopyridoxyl)amines, overview, no activity with pyridoxamine, N-(pyridoxyl)-glycine, N-(pyridoxyl)-S-alanine, or N-(pyridoxyl)-L-tyrosine Oryctolagus cuniculus ?
-
 ?
N10-(5'-phosphopyridoxyl)-1,10-diaminodecane + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + 1,10-diaminodecane + H2O2
-
 ?
pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxine 5'-phosphate + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Oryctolagus cuniculus