Any feedback?
Literature summary for 1.4.3.5 extracted from
- Immobilization of yeast pyridoxamine (pyridoxine) 5-phosphate oxidase by organomercurial agarose (1978), FEBS Lett., 93, 331-334.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
pyridoxine 5'-phosphate | immobilized enzyme | Saccharomyces cerevisiae |  |
| 0.0027 | - |
pyridoxine 5'-phosphate | soluble enzyme | Saccharomyces cerevisiae | |
| 0.01 | - |
pyridoxamine 5'-phosphate | immobilized enzyme | Saccharomyces cerevisiae | |
| 0.018 | - |
pyridoxamine 5'-phosphate | soluble enzyme | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| approx. 25°C, immobilized enzyme, 200 mM K3PO4, pH 7.0, longer than 1 week | Saccharomyces cerevisiae |
| approx. 5°C, immobilized enzyme, 200 mM K3PO4, pH 7.0, longer than 1 month | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxamine 5'-phosphate + H2O + O2 | - |
Saccharomyces cerevisiae | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
soluble enzyme | Saccharomyces cerevisiae |
| 55 | - |
immobilized enzyme | Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | at 30°C: about 50% of maximal activity, at 55°C: about 15% of maximal activity | Saccharomyces cerevisiae |
| 38 | 60 | at 38°C: about 55% of maximal activity, at 60°C: about 20% of maximal activity | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
half-life: 4 days immobilized enzyme, less than 1 day free enzyme | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
pyridoxine 5'-phosphate, immobilized enzyme | Saccharomyces cerevisiae |
| 8 | - |
soluble enzyme | Saccharomyces cerevisiae |
| 8 | - |
pyridoxine 5'-phosphate | Saccharomyces cerevisiae |
| 9 | - |
pyridoxamine 5'-phosphate, soluble and immobilized enzyme | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
