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Literature summary for 1.4.3.4 extracted from

  • Li, M.; Binda, C.; Mattevi, A.; Edmondson, D.E.
    Functional role of the "aromatic cage" in human monoamine oxidase B: structures and catalytic properties of Tyr435 mutant proteins (2006), Biochemistry, 45, 4775-4784.
    View publication on PubMed
Search for more literature for 1.4.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes in Pichia pastoris Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor diffusion method, mutant enzymes Y435H, Y435F, Y435L, Y435W and wild-type enzyme in complex with p-nitrobenzylamine Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y435F kcat/KM for benzylamine is 21.3fold lower than wild-type value, kcat/KM for p-trifluoromethyl-benzylamine is 40fold lower than wild-type value, kcat/KM for p-nitrobenzylamine is 4.1fold lower than wild-type value, kcat/KM for phenylethylamine is 35.9fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 8.3fold lower than wild-type value Homo sapiens
Y435H kcat/KM for benzylamine is 120fold lower than wild-type value, kcat/KM for p-trifluoromethyl-benzylamine is 13fold lower than wild-type value, kcat/KM for p-nitrobenzylamine is 4.4fold lower than wild-type value, kcat/KM for phenylethylamine is 1037fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 45.2 fold lower than wild-type value Homo sapiens
Y435L kcat/KM for benzylamine is 128fold lower than wild-type value, kcat/KM for p-trifluoromethyl-benzylamine is 58fold lower than wild-type value, kcat/KM for phenylethylamine is 15.2fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 2.7fold lower than wild-type value Homo sapiens
Y435W kcat/KM for benzylamine is 9524fold lower than wild-type value, kcat/KM for phenylethylamine is 13889fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 23fold lower than wild-type value Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
p-nitrobenzylamine substrate for wild-type enzyme, competitive inhibitor for mutant enzyme Y435L Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 p-nitrophenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.013
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
0.016
-
 2-Phenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.05
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.05
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
0.05
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
0.09
-
 p-nitrobenzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.13
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
0.14
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
0.15
-
 benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.22
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
0.29
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
0.49
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
0.68
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
0.76
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
0.99
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.15
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
2
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
2.49
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
7.2
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
8.8
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27338
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethylamine + H2O + O2
-
 Homo sapiens 2-phenylethanal + NH3 + H2O2
-
 ?
benzylamine + H2O + O2
-
 Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
p-nitrobenzylamine + H2O + O2 poor substrate for wild-type enzyme and mutant enzymes Y435H and Y435F Homo sapiens 4-nitrobenzaldehyde + NH3 + H2O2
-
 ?
p-nitrophenylethylamine + H2O + O2 good substrate for wild-type and mutant enzymes Y435H, Y435F, Y435L and Y435W Homo sapiens 4-nitrophenylacetaldehyde + NH3 + H2O2
-
 ?
p-trifluoromethyl-benzylamine + H2O + O2
-
 Homo sapiens 4-trifluoromethylbenzaldehyde + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
monoamine oxidase B
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
0.028
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
0.066
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
0.185
-
 p-nitrobenzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.23
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
0.38
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
0.455
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
0.647
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
0.79
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
0.87
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.33
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.33
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.8
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.8
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.8
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.85
-
 p-nitrophenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.99
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
2.23
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
2.24
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
2.85
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
3.82
-
 2-Phenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
5
-
 benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
6.08
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens