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Literature summary for 1.4.3.4 extracted from

  • Li, M.; Hubalek, F.; Newton-Vinson, P.; Edmondson, D.E.
    High-level expression of human liver monoamine oxidase A in Pichia pastoris: comparison with the enzyme expressed in Saccharomyces cerevisiae (2002), Protein Expr. Purif., 24, 152-162.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
advantage of the expression in Pichia pastoris compared with the expression in Saccharomyces cerevisiae: a higher level of homogeneity of the isolated enzyme Homo sapiens
expression in Pichia pastoris Homo sapiens
expression in Saccharomyces cerevisiae Homo sapiens
high-level expression in Pichia pastoris, higher expression level than in Saccharomyces cerevisiae and higher level of homogeneity of the isolated enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Clorgyline
-
Homo sapiens
N-(2-aminoethyl)-aryl-carboxamide
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information comparison of Km of MAO expressed in Saccharomyces cerevisiae and Pichia pastoris Homo sapiens
0.89
-
phenethylamine 11°C Homo sapiens
1.36
-
dopamine 11°C Homo sapiens
2.92
-
serotonin 11°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60512
-
x * 60512, electrospray mass spectroscopy, including one covalent FAD, MAO-A Homo sapiens
60512
-
x * 60512, electrospray mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P21397
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.76
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, in 50% glycerol v/v for up to 5 months without measurable loss of activity Homo sapiens
4°C, enzyme desalted by G-25 gel filtration, stable for 30 days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethylamine + H2O + O2
-
Homo sapiens 2-phenylethanal + NH3 + H2O2
-
?
dopamine + H2O + O2
-
Homo sapiens (3,4-dihydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
kynuramine + H2O + O2
-
Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
?
RCH2NH2 + H2O + O2
-
Homo sapiens RCHO + NH3 + H2O2
-
?
serotonin + H2O + O2
-
Homo sapiens (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 60512, electrospray mass spectroscopy, including one covalent FAD, MAO-A Homo sapiens
? x * 60512, electrospray mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
MAO-A
-
Homo sapiens
monoamine oxidase A
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15
-
below, enzyme expressed in Saccharomyces cerevisiae, 60 min stable Homo sapiens
20
-
enzyme expressed in Pichia pastoris, 60 min stable Homo sapiens
20
-
enzyme expressed in Saccharomyces cerevisiae, 60 min 15% loss of activity Homo sapiens
25
-
enzyme expressed in Pichia pastoris, 60 min, 8% loss of activity Homo sapiens
25
-
enzyme expressed in Saccharomyces cerevisiae, 60 min 50% loss of activity Homo sapiens
30
-
enzyme expressed in Pichia pastoris, 60 min, 60% loss of activity Homo sapiens
30
-
enzyme expressed in Saccharomyces cerevisiae, 60 min 80% loss of activity Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.249
-
dopamine 11°C Homo sapiens
0.426
-
serotonin 11°C Homo sapiens
1.481
-
phenethylamine 11°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
8alpha-S-cysteinyl-FAD 1 mol per mol of enzyme Homo sapiens
flavin MAO-A contain 1 mol of 8-alpha-S-cysteinyl 406 FAD per mol Homo sapiens