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Literature summary for 1.4.3.3 extracted from

  • Saam, J.; Rosini, E.; Molla, G.; Schulten, K.; Pollegioni, L.; Ghisla, S.
    O2 reactivity of flavoproteins: dynamic access of dioxygen to the active site and role of a H+ relay system in D-amino acid oxidase (2010), J. Biol. Chem., 285, 24439-24446.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.3

Protein Variants

Protein Variants Comment Organism
G52V site-directed mutagenesis, in the mutant the reactivity of the reduced enzyme with O2 is decreased about 100fold and the turnover number about 1000fold compared to the wild-type enzyme Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information reaction kinetics of oxidative and reductive half-reactions of wild-type and mutant enzymes, overview Rhodotorula toruloides
0.036
-
 D-alanine pH 8.5, 15°C, mutant G52V Rhodotorula toruloides
0.15
-
 O2 pH 8.5, 15°C, mutant G52V Rhodotorula toruloides
2.6
-
 D-alanine pH 8.5, 15°C, wild-type enzyme Rhodotorula toruloides
3
-
 O2 pH 8.5, 15°C, wild-type enzyme Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-alanine + H2O + O2 Rhodotorula toruloides
-
 pyruvate + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides P80324
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
additional information specific dynamic channel for the diffusion of O2 leads from solvent to the flavin Si-side. O2 affinity, catalytic center, and substrate binding of wild-type and mutant enzymes, molecular dynamics simulations built from the three-dimensional structure, PDB ID 1c0p, in which the ligand D-Ala is replaced by imino pyruvate, overview Rhodotorula toruloides ?
-
 ?

Synonyms

Synonyms Comment Organism
DAAO
-
 Rhodotorula toruloides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 assay at Rhodotorula toruloides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
 D-alanine pH 8.5, 15°C, mutant G52V Rhodotorula toruloides
330
-
 D-alanine pH 8.5, 15°C, wild-type enzyme Rhodotorula toruloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Rhodotorula toruloides

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rhodotorula toruloides