Cloned (Comment) | Organism |
---|---|
two cDNA genes were connected by a hexanucleotide linker and heterologously expressed in Escherichia coli to produce the corresponding double DAO with two subunits fused into a single polypeptide | Rhodotorula toruloides |
two cDNA genes were connected by a hexanucleotide linker and heterologously expressed in Escherichia coli to produce the corresponding double DAO with two subunits fused into a single polypeptide | Trigonopsis variabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-alanine | native enzyme, at 37°C | Rhodotorula toruloides | |
3 | - |
D-alanine | native enzyme, at 37°C | Trigonopsis variabilis | |
6 | - |
D-alanine | double fusion enzyme, at 37°C | Rhodotorula toruloides | |
6 | - |
cephalosporin C | native enzyme, at 37°C | Rhodotorula toruloides | |
9 | - |
cephalosporin C | native enzyme, at 37°C | Trigonopsis variabilis | |
13 | - |
D-alanine | double fusion enzyme, at 37°C | Trigonopsis variabilis | |
13 | - |
cephalosporin C | double fusion enzyme, at 37°C | Rhodotorula toruloides | |
20 | - |
cephalosporin C | double fusion enzyme, at 37°C | Trigonopsis variabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000, SDS-PAGE | Rhodotorula toruloides |
20000 | - |
2 * 20000, SDS-PAGE | Trigonopsis variabilis |
40000 | - |
SDS-PAGE | Rhodotorula toruloides |
40000 | - |
SDS-PAGE | Trigonopsis variabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | - |
- |
- |
Trigonopsis variabilis | - |
- |
- |
Oxidation Stability | Organism |
---|---|
double DAO exhibits a half-life of about 30 min in the presence of 10 mM H2O2 which is 1.5fold longer compared to the native DAO | Trigonopsis variabilis |
double DAO exhibits a half-life of about 60 min in the presence of 10 mM H2O2 which is 2fold longer compared to the native DAO | Rhodotorula toruloides |
Purification (Comment) | Organism |
---|---|
His-Bind column chromatography | Rhodotorula toruloides |
His-Bind column chromatography | Trigonopsis variabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72 | - |
native enzyme, with D-alanine as substrate, at 37°C | Rhodotorula toruloides |
77 | - |
double fusion enzyme, with D-alanine as substrate, at 37°C | Rhodotorula toruloides |
95 | - |
native enzyme, with D-alanine as substrate, at 37°C | Trigonopsis variabilis |
96 | - |
double fusion enzyme, with D-alanine as substrate, at 37°C | Trigonopsis variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | 14% activity compared to D-alanine | Trigonopsis variabilis | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
cephalosporin C + H2O + O2 | 36% activity compared to D-alanine | Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | 100% activity | Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | 100% activity | Trigonopsis variabilis | pyruvate + NH3 + H2O2 | - |
? | |
D-arginine + H2O + O2 | 19% activity compared to D-alanine | Rhodotorula toruloides | 5-guanidino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-arginine + H2O + O2 | 42% activity compared to D-alanine | Trigonopsis variabilis | 5-guanidino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-asparagine + H2O + O2 | 38% activity compared to D-alanine | Rhodotorula toruloides | 2-oxosuccinamic acid + NH3 + H2O2 | - |
? | |
D-asparagine + H2O + O2 | 67% activity compared to D-alanine | Trigonopsis variabilis | 2-oxosuccinamic acid + NH3 + H2O2 | - |
? | |
D-aspartate + H2O + O2 | 2% activity compared to D-alanine | Trigonopsis variabilis | oxaloacetate + NH3 + H2O2 | - |
? | |
D-aspartate + H2O + O2 | 3% activity compared to D-alanine | Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? | |
D-cysteine + H2O + O2 | 18% activity compared to D-alanine | Rhodotorula toruloides | 2-oxo-3-thiopropionic acid + NH3 + H2O2 | - |
? | |
D-cysteine + H2O + O2 | 9% activity compared to D-alanine | Trigonopsis variabilis | 2-oxo-3-thiopropionic acid + NH3 + H2O2 | - |
? | |
D-glutamate + H2O + O2 | 3% activity compared to D-alanine | Rhodotorula toruloides | alpha-ketoglutarate + NH3 + H2O2 | - |
? | |
D-glutamate + H2O + O2 | 9% activity compared to D-alanine | Trigonopsis variabilis | alpha-ketoglutarate + NH3 + H2O2 | - |
? | |
D-glutamine + H2O + O2 | 58% activity compared to D-alanine | Rhodotorula toruloides | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
D-glutamine + H2O + O2 | 78% activity compared to D-alanine | Trigonopsis variabilis | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
D-histidine + H2O + O2 | 60% activity compared to D-alanine | Rhodotorula toruloides | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
D-histidine + H2O + O2 | 69% activity compared to D-alanine | Trigonopsis variabilis | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
D-isoleucine + H2O + O2 | 60% activity compared to D-alanine | Rhodotorula toruloides | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-isoleucine + H2O + O2 | 69% activity compared to D-alanine | Trigonopsis variabilis | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-leucine + H2O + O2 | 37% activity compared to D-alanine | Trigonopsis variabilis | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-leucine + H2O + O2 | 86% activity compared to D-alanine | Rhodotorula toruloides | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-lysine + H2O + O2 | 7% activity compared to D-alanine | Trigonopsis variabilis | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
D-lysine + H2O + O2 | 8% activity compared to D-alanine | Rhodotorula toruloides | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
D-methionine + H2O + O2 | 113% activity compared to D-alanine | Rhodotorula toruloides | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-methionine + H2O + O2 | 77% activity compared to D-alanine | Trigonopsis variabilis | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | 44% activity compared to D-alanine | Trigonopsis variabilis | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | 94% activity compared to D-alanine | Rhodotorula toruloides | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | 21% activity compared to D-alanine | Trigonopsis variabilis | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | 46% activity compared to D-alanine | Rhodotorula toruloides | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-serine + H2O + O2 | 23% activity compared to D-alanine | Trigonopsis variabilis | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
D-serine + H2O + O2 | 49% activity compared to D-alanine | Rhodotorula toruloides | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
D-threonine + H2O + O2 | 18% activity compared to D-alanine | Rhodotorula toruloides | 2-oxo-3-hydroxybutyric acid + NH3 + H2O2 | - |
? | |
D-threonine + H2O + O2 | 2% activity compared to D-alanine | Trigonopsis variabilis | 2-oxo-3-hydroxybutyric acid + NH3 + H2O2 | - |
? | |
D-tryptophan + H2O + O2 | 129% activity compared to D-alanine | Trigonopsis variabilis | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
D-tryptophan + H2O + O2 | 92% activity compared to D-alanine | Rhodotorula toruloides | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
D-tyrosine + H2O + O2 | 18% activity compared to D-alanine | Rhodotorula toruloides | 4-hydroxyphenylpyruvic acid + NH3 + H2O2 | - |
? | |
D-tyrosine + H2O + O2 | 19% activity compared to D-alanine | Trigonopsis variabilis | 4-hydroxyphenylpyruvic acid + NH3 + H2O2 | - |
? | |
D-valine + H2O + O2 | 60% activity compared to D-alanine | Rhodotorula toruloides | alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
D-valine + H2O + O2 | 63% activity compared to D-alanine | Trigonopsis variabilis | alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
glycine + 2 H2O + O2 | 2% activity compared to D-alanine | Trigonopsis variabilis | 2 formic acid + NH3 + H2O2 | - |
? | |
glycine + 2 H2O + O2 | 3% activity compared to D-alanine | Rhodotorula toruloides | 2 formic acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 20000, SDS-PAGE | Rhodotorula toruloides |
homodimer | 2 * 20000, SDS-PAGE | Trigonopsis variabilis |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Rhodotorula toruloides |
D-amino acid oxidase | - |
Trigonopsis variabilis |
DAAO | - |
Rhodotorula toruloides |
DAAO | - |
Trigonopsis variabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
double fusion enzyme | Rhodotorula toruloides |
55 | - |
native enzyme | Rhodotorula toruloides |
60 | - |
double fusion enzyme | Trigonopsis variabilis |
65 | - |
native enzyme | Trigonopsis variabilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | retains more than 80% of the maximal activity after incubation for 60 min at 30-60°C | Rhodotorula toruloides |
37 | 60 | retains more than 80% of the maximal activity after incubation for 60 min at 37-60°C | Trigonopsis variabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
cephalosporin C | native enzyme, at 37°C | Trigonopsis variabilis | |
60 | - |
D-alanine | native enzyme, at 37°C | Rhodotorula toruloides | |
64 | - |
cephalosporin C | double fusion enzyme, at 37°C | Trigonopsis variabilis | |
70 | - |
cephalosporin C | native enzyme, at 37°C | Rhodotorula toruloides | |
74 | - |
D-alanine | native enzyme, at 37°C | Trigonopsis variabilis | |
78 | - |
D-alanine | double fusion enzyme, at 37°C | Rhodotorula toruloides | |
80 | - |
cephalosporin C | double fusion enzyme, at 37°C | Rhodotorula toruloides | |
88 | - |
D-alanine | double fusion enzyme, at 37°C | Trigonopsis variabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
native and double fusion enzymes | Rhodotorula toruloides |
8.5 | - |
native and double fusion enzymes | Trigonopsis variabilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | retains more than 80% of the maximal activity after incubation for 60 min at pH 5-10 | Rhodotorula toruloides |
5 | 10 | retains more than 80% of the maximal activity after incubation for 60 min at pH 5-10 | Trigonopsis variabilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodotorula toruloides | |
FAD | - |
Trigonopsis variabilis |