Protein Variants | Comment | Organism |
---|---|---|
Y238F | 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 4.7fold lower than the wild-type value, Km-value for D-Pro is 1.7fold lower than the wild-type value, Km-value for D-Trp is 1.5fold lower than the wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3fold lower than the wild-type value, Km-value for D-Phe is 4.3fold lower than the wild-type value | Rhodotorula toruloides |
Y238S | 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 8fold lower than the wild-type value, Km-value for D-Pro is 1.6fold lower than the wild-type value, Km-value for D-Trp is identical to wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3.2fold lower than the wild-type value, Km-value for D-Phe is 7.5fold lower than the wild-type value | Rhodotorula toruloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoate | competitive | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-Phe | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
0.07 | - |
D-Phe | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
0.2 | - |
D-Trp | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
0.26 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
0.3 | - |
D-Trp | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
0.3 | - |
D-Phe | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
0.3 | - |
D-Trp | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
0.96 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
1.7 | - |
D-Ser | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.9 | - |
cephalosporin C | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
1.9 | - |
cephalosporin C | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
2.3 | - |
O2 | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
2.6 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
2.9 | - |
D-Ser | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
5 | - |
cephalosporin C | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
6 | - |
D-Val | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
6.1 | - |
D-Val | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
7.5 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
7.8 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
12.3 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
13.5 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
13.7 | - |
D-Ser | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
18.9 | - |
D-Val | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
21.5 | - |
D-Pro | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | P80324 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
D-Ala + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-Phe + H2O + O2 | - |
Rhodotorula toruloides | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-Pro + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-Ser + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
D-Trp + H2O + O2 | - |
Rhodotorula toruloides | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
D-Val + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoisovalerate + NH3 + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
120 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238S | Rhodotorula toruloides | |
125 | - |
O2 | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
125 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme Y238F | Rhodotorula toruloides | |
350 | - |
O2 | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
350 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides |