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Literature summary for 1.4.3.25 extracted from

  • Matsui, D.; Terai, A.; Asano, Y.
    L-Arginine oxidase from Pseudomonas sp. TPU 7192: Characterization, gene cloning, heterologous expression, and application to L-arginine determination (2016), Enzyme Microb. Technol., 82, 151-157.
    View publication on PubMed
Search for more literature for 1.4.3.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.149
-
 L-arginine pH 7.0, 37°C Pseudomonas sp.
5.25
-
 L-lysine pH 7.0, 37°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
-
 Pseudomonas sp.
66300
-
-
 Pseudomonas sp.
528000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. HW613270
-
-
Pseudomonas sp. TPU 7192 HW613270
-
-

Purification (Commentary)

Purification (Comment) Organism
both native and recombinant protein Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O + O2
-
 Pseudomonas sp. 5-guanidino-2-oxopentanoate + NH3 + H2O2 the enzyme catalyzes the oxidative deamination of L-arginine and converts L-arginine to 2-ketoarginine, which is nonenzymatically converted into 4-guanidinobutyric acid when the hydrogen peroxide formed by L-arginine oxidation is not removed ?
L-arginine + H2O + O2
-
 Pseudomonas sp. TPU 7192 5-guanidino-2-oxopentanoate + NH3 + H2O2 the enzyme catalyzes the oxidative deamination of L-arginine and converts L-arginine to 2-ketoarginine, which is nonenzymatically converted into 4-guanidinobutyric acid when the hydrogen peroxide formed by L-arginine oxidation is not removed ?
L-canavanine + H2O + O2
-
 Pseudomonas sp. 2-oxocanavanine + NH3 + H2O2
-
 ?
L-canavanine + H2O + O2
-
 Pseudomonas sp. TPU 7192 2-oxocanavanine + NH3 + H2O2
-
 ?
L-homoarginine + H2O + O2
-
 Pseudomonas sp. 2-oxohomoarginine + NH3 + H2O2
-
 ?
L-homoarginine + H2O + O2
-
 Pseudomonas sp. TPU 7192 2-oxohomoarginine + NH3 + H2O2
-
 ?
L-lysine + H2O + O2
-
 Pseudomonas sp. 2-oxolysine + NH3 + H2O2
-
 ?
L-lysine + H2O + O2
-
 Pseudomonas sp. TPU 7192 2-oxolysine + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 66000, SDS-PAGE, 8 * 66300, calculated Pseudomonas sp.

Synonyms

Synonyms Comment Organism
aroD
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol of FAD being contained per mol of subunit. Purified enzyme shows absorption maxima at 275, 375, and 460 nm Pseudomonas sp.