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Literature summary for 1.4.3.24 extracted from

  • Qiu, J.; Ma, Y.; Wen, Y.; Chen, L.; Wu, L.; Liu, W.
    Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine (2012), Appl. Environ. Microbiol., 78, 2154-2160.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition Pseudomonas sp.
Co2+ strong inhibition Pseudomonas sp.
Cu2+ strong inhibition Pseudomonas sp.
additional information not inhibitory: Ca2+, Mg2+, Mn2+, Ni2+, Zn2+ Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.247
-
 4-(methylamino)-1-(pyridin-3-yl)butan-1-one pH 8.0, temperature not specified in the publication Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54101
-
 x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein Pseudomonas sp.
55000
-
 x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. H8ZPX1
-
-
Pseudomonas sp. HZN6 H8ZPX1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2
-
 Pseudomonas sp. 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2
-
 ?
4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2
-
 Pseudomonas sp. HZN6 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2
-
 ?
additional information no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine Pseudomonas sp. ?
-
 ?
additional information no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine Pseudomonas sp. HZN6 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein Pseudomonas sp.

Synonyms

Synonyms Comment Organism
PAO
-
 Pseudomonas sp.
pseudooxynicotine amine oxidase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
151
-
 4-(methylamino)-1-(pyridin-3-yl)butan-1-one pH 8.0, temperature not specified in the publication Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound, 0.5 mol per mol of subunit Pseudomonas sp.
additional information no FMN detected Pseudomonas sp.

General Information

General Information Comment Organism
physiological function gene disruption mutant strain is unable to use nicotine as the sole carbon source and accumulates pseudooxynicotine Pseudomonas sp.