Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2.94% residual activity at 0.5 mM | Vicia faba | |
aminoguanidine | 3.92% residual activity at 0.25 mM | Vicia faba | |
Cu2+ | 7.84% residual activity at 3 mM | Vicia faba | |
EDTA-Na2 | 76.47% residual activity at 1 mM | Vicia faba | |
EGTA | 71.57% residual activity at 1 mM | Vicia faba | |
Fe3+ | 10.78% residual activity at 3 mM | Vicia faba | |
L-cysteine | complete inhibition at 0.1 mM | Vicia faba | |
Mg2+ | 78.43% residual activity at 3 mM | Vicia faba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
putrescine | at pH 6.5 and 40°C | Vicia faba | |
0.96 | - |
spermidine | at pH 6.5 and 40°C | Vicia faba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Ca2+, Zn2+, Mn2+, and Ba2+ | Vicia faba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Vicia faba |
121000 | - |
gel filtration | Vicia faba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vicia faba | - |
cultivar Qidou 2 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration | Vicia faba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Vicia faba | - |
shoot | - |
Vicia faba | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the crude extract has a specific activity of 9.1 units/mg, while the 215fold purified enzyme has a specific activity of 1962.47 units/mg. A change in absorbance at 555 nm of 0.01 per minute is considered as one unit of enzyme activity | Vicia faba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not degrade spermine | Vicia faba | ? | - |
? | |
putrescine + H2O + O2 | - |
Vicia faba | ? | - |
? | |
spermidine + H2O + O2 | - |
Vicia faba | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 52000, SDS-PAGE | Vicia faba |
Synonyms | Comment | Organism |
---|---|---|
DAO | - |
Vicia faba |
EC 1.4.3.6 | formerly | Vicia faba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vicia faba |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme retains its activity after being incubated at 30°C for 30 min, the enzyme is rapidly deactivated above 45°C and reaches almost zero activity at 60°C | Vicia faba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Vicia faba |
Organism | Comment | Expression |
---|---|---|
Vicia faba | enzyme activity in the shoot is undetectable up to 2 days of germination but then increases significantly | up |
General Information | Comment | Organism |
---|---|---|
metabolism | diamine oxidase is one of the key enzymes for gamma-aminobutyric acid formation | Vicia faba |